697 Result(s)

Article

Amino acid and vitamin requirements in mammalian cultured cells

The development of the tissue culture technique has enabled us to cultivate mammalian cells in a way which is similar to that in use with bacterial cells. As such, the nutritional requirements of mammalian cel...

K. Yamamoto, A. Niwa MD in Amino Acids (1993)

Article

Conscientious metabolic monitoring on a patient with hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome undergoing anaesthesia

Currently we know not more than 50 patients who show an interesting combination of increased plasma ornithine concentrations, postprandial hyperammonemia, and homocitrullinuria (HHH-syndrome). Since exact kno...

J. Mühling, M. G. Dehne, M. Fuchs, A. Sablotzki, S. Weiss, J. Spatz… in Amino Acids (2001)

Article

Protein oxidation at the air-lung interface

 Whilst performing its normal functions the lung is required to deal with a range of toxic insults. Whether these are infectious agents, allergens or air pollutants they subject the lung to a range of direct a...

F. J. Kelly, I. S. Mudway in Amino Acids (2003)

Article

Using cellular automata to generate image representation for biological sequences

A novel approach to visualize biological sequences is developed based on cellular automata (Wolfram, S. Nature 1984, 311, 419–424), a set of discrete dynamical systems in which space and time are discrete. By tra...

X. **ao, S. Shao, Y. Ding, Z. Huang, X. Chen, K.-C. Chou in Amino Acids (2005)

Article

Using string kernel to predict signal peptide cleavage site based on subsite coupling model

M. Wang, J. Yang, K.-C. Chou in Amino Acids (2005)

Article

Using cellular automata images and pseudo amino acid composition to predict protein subcellular location

The avalanche of newly found protein sequences in the post-genomic era has motivated and challenged us to develop an automated method that can rapidly and accurately predict the localization of an uncharacteri...

X. **ao, S. Shao, Y. Ding, Z. Huang, K.-C. Chou in Amino Acids (2006)

Article

Anti-SARS drug screening by molecular docking

Starting from a collection of 1386 druggable compounds obtained from the 3D pharmacophore search, we performed a similarity search to narrow down the scope of docking studies. The template molecule is KZ7088 (...

D.-Q. Wei, R. Zhang, Q.-S. Du, W.-N. Gao, Y. Li, H. Gao, S.-Q. Wang… in Amino Acids (2006)

Article

Intracellular cytokine optimization and standard operating procedure

We describe here a method for optimizing the use of polychromatic flow cytometry (with up to 17 fluorochromes simultaneously) in surface and intracellular staining of human T lymphocytes. We will highlight and...

Laurie Lamoreaux, Mario Roederer, Richard Koup in Nature Protocols (2006)

Article

pC6-2/caspase-6 system to purify glutathione-S-transferase-free recombinant fusion proteins expressed in Escherichia coli

Glutathione-S-transferase (GST) fusion protein expression vectors are often employed for the expression and purification of proteins in Escherichia coli. GST is then removed by site-specific proteolysis using thr...

Prabhat Kumar Purbey, P Cyril Jayakumar, Milind S Patole… in Nature Protocols (2006)

Article

A continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis

This protocol describes a streamlined method of plasmid DNA extraction by continual thermal lysis, a modification of the basic boiling lysis technique, to simplify the processing of large volumes of Escherichia c...

Kaichun Zhu, Huali **, Zhonghuai He, Qinghong Zhu, Bin Wang in Nature Protocols (2006)

Article

Programmable reactivity-based one-pot oligosaccharide synthesis

A detailed protocol is described for the application of a programmable one-pot oligosaccharide synthesis methodology to the synthesis of fucosyl GM1. This serves as a general example of the application of this...

**q-Chyi Lee, William A Greenberg, Chi-Huey Wong in Nature Protocols (2006)

Article

Primary culture of Caenorhabditis elegans develo** embryo cells for electrophysiological, cell biological and molecular studies

Cell culture is an invaluable tool for investigation of basic biological processes. However, technical hurdles including low cell yield, poor cell differentiation and poor attachment to the growth substrate ha...

Kevin Strange, Michael Christensen, Rebecca Morrison in Nature Protocols (2007)

Article

Selective proliferation of rat hepatocyte progenitor cells in serum-free culture

This protocol details a method of obtaining selectively proliferated hepatocyte progenitor cells using hyaluronic acid (HA)–coated dishes and serum-free medium. A small hepatocyte (SH) is a hepatocyte progenit...

Qijie Chen, Junko Kon, Hidekazu Ooe, Kazunori Sasaki, Toshihiro Mitaka in Nature Protocols (2007)

Article

A dynamic model for the p53 stress response networks under ion radiation

P53 controls the cell cycle arrest and cell apoptosis through interaction with the downstream genes and their signal pathways. To stimulate the investigation into the complicated responses of p53 under the cir...

J.-P. Qi, S.-H. Shao, D.-D. Li, G.-P. Zhou in Amino Acids (2007)

Article

Virtual screening for finding natural inhibitor against cathepsin-L for SARS therapy

Recently Simmons et al. reported a new mechanism for SARS virus entry into target cells, where MDL28170 was identified as an efficient inhibitor of CTSL-meditated substrate cleavage with IC₅₀ of 2.5 nmol/l. Based...

S.-Q. Wang, Q.-S. Du, K. Zhao, A.-X. Li, D.-Q. Wei, K.-C. Chou in Amino Acids (2007)

Article

Purification of antibodies using the synthetic affinity ligand absorbent MAbsorbent A2P

A protocol for the purification of polyclonal antibodies from ovine serum using the synthetic protein A absorbent MAbsorbent A2P is described. Clarified serum is loaded directly onto the affinity column withou...

Sunil Chhatre, Nigel J Titchener-Hooker, Anthony R Newcombe… in Nature Protocols (2007)

Article

Multi-layered network structure of amino acid (AA) metabolism characterized by each essential AA-deficient condition

The concentrations of free amino acids in plasma change coordinately and their profiles show distinctive features in various physiological conditions; however, their behavior can not always be explained by the...

N. Shikata, Y. Maki, Y. Noguchi, M. Mori, T. Hanai, M. Takahashi, M. Okamoto in Amino Acids (2007)

Article

Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research

In transmission electron microscopy (TEM), electrons are transmitted through a plastic-embedded specimen, and an image is formed. TEM enables the resolution and visualization of detail not apparent via light m...

Lesley Graham, Jan Marc Orenstein in Nature Protocols (2007)

Article

Prediction of mutations engineered by randomness in H5N1 neuraminidases from influenza A virus

In this proof-of-concept study, we attempt to determine whether the cause-mutation relationship defined by randomness is protein dependent by predicting mutations in H5N1 neuraminidases from influenza A virus,...

G. Wu, S. Yan in Amino Acids (2008)

Article

Regulation of aspartate-derived amino acid homeostasis in potato plants (Solanum tuberosum L.) by expression of E. coli homoserine kinase

The availability of the carbon backbone O-phosphohomoserine (OPHS) is critical to methionine (met) and threonine (thr) synthesis. OPHS derives from homoserine and is formed by homoserine kinase (HSK). To clarify ...

J. Rinder, A. P. Casazza, R. Hoefgen, H. Hesse in Amino Acids (2008)