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Article
The mRNA for an ETR1 homologue in tomato is constitutively expressed in vegetative and reproductive tissues
Dominant mutations in the Arabidopsis ETR1 gene block the ethylene signal transduction pathway. The ETR1 gene has been cloned and sequenced. Using the ETR1 cDNA as a probe, we identified a cDNA homologue (eTAE1) ...
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Article
Open AccessA protein knockdown strategy to study the function of β-catenin in tumorigenesis
The Wnt signaling pathway plays critical roles in cell proliferation and cell fate determination at many stages of development. A critical downstream target of Wnt signaling is the cytosolic β-catenin, which i...
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Protocol
Determining Protein Half-Lives
Controlling the stability of cellular proteins is a fundamental way by which cells regulate growth, differentiation, survival, and development. Measuring the turnover rate of a protein is often the first step ...
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Article
Open AccessTransient in uteroknockout (TIUKO) of C-MYC affects late lung and intestinal development in the mouse
Developmentally important genes often result in early lethality in knockout animals. Thus, the direct role of genes in late gestation organogenesis cannot be assessed directly. In utero delivery of transgenes was...
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Chapter and Conference Paper
New Fast Detection Method of Forest Fire Monitoring and Application Based on FY-1D/MVISR Data
In this paper, the authors proposed a new work and process flow algorithm about remote sensing image data to forest fire identification and monitoring, which was greatly different with the traditional approach...
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Article
High glucose condition upregulated Txnip expression level in rat mesangial cells through ROS/MEK/MAPK pathway
Thioredoxin interacting protein (Txnip) is one of the most abundantly up-regulated genes in response to hyperglycemia. The increased renal expression of Txnip was associated with type IV collagen accumulation ...
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Article
Maximizing target protein ablation by integration of RNAi and protein knockout
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Article
Open AccessTranscription analysis on response of swine lung to H1N1 swine influenza virus
As a mild, highly contagious, respiratory disease, swine influenza always damages the innate immune systems, and increases susceptibility to secondary infections which results in considerable morbidity and mor...
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Article
Stoichiometric traits of oriental oak (Quercus variabilis) acorns and their variations in relation to environmental variables across temperate to subtropical China
The stoichiometric composition of plant reproductive organs varies with environmental conditions at a large geographical scale, which has potentially important consequences both for plant reproductive success ...
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Article
Transmission of important chromosomal regions under selection revealed in rice pedigree breeding programs
Genetic analysis across a whole plant genome based on pedigree information offers considerable potential for enhancing genetic gain from plant breeding programs through quantitative trait loci (QTL) map** an...
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Article
Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis
Mutations of the CUL4B ubiquitin ligase gene are causally linked to syndromic X-linked mental retardation (XLMR). However, the pathogenic role of CUL4B mutations in neuronal and developmental defects is not under...
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Article
Characterization and constitutive expression of a novel endo-1,4-β-d-xylanohydrolase from Aspergillus niger in Pichia pastoris
A putative endo-1,4-β-d-xylanohydrolase gene xyl10 from Aspergillus niger, encoding a 308-residue mature xylanase belonging to glycosyl hydrolase family 10, was constitutively expressed in Pichia pastoris. The re...
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Article
Characterization and constitutive expression of an acidic mesophilic endo-1,4-β-d-xylanohydrolase with high thermotolerance and catalytic efficiency in Pichia pastoris
A putative endo-1,4-β-d-xylanohydrolase gene xyl11 from Aspergillus niger, encoding a 188-residue xylanase of glycosyl hydrolase family 11, was constitutively expressed in Pichia pastoris. The recombinant Xyl11 e...
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Article
Open AccessCHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2
NS2 from influenza A virus mediates Crm1-dependent vRNP nuclear export through interaction with Crm1. However, even though the nuclear export signal 1 (NES1) of NS2 does not play a requisite role in NS2–Crm1 i...
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Article
Expression of Critical Sulfur- and Iron-Oxidation Genes and the Community Dynamics During Bioleaching of Chalcopyrite Concentrate by Moderate Thermophiles
Sulfate adenylyltransferase gene and 4Fe–4S ferredoxin gene are the key genes related to sulfur and iron oxidations during bioleaching system, respectively. In order to better understand the bioleaching and mi...
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Article
Warfarin traps human vitamin K epoxide reductase in an intermediate state during electron transfer
Mass spectrometry and biochemical analyses reveal that the major form of VKOR found in cells features a disulfide bond between Cys51 and Cys132, and this intermediate is the target of the anticoagulant drug wa...
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Article
Open AccessCharacterization of enzymatic properties of two novel enzymes, 3,4-dihydroxyphenylacetate dioxygenase and 4-hydroxyphenylacetate 3-hydroxylase, from Sulfobacillus acidophilus TPY
As an environmental pollutant, 4-hydroxyphenylacetate (4-HPA) was a product of softwood lignin decomposition and was found in industrial effluents from olive oil production. Sulfobacillus acidophilus TPY was a mo...
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Article
Immune responses in Brazilian banana determining the pathogenic differences between the physiological races 1 and 4 of Fusarium oxysporum f. sp. cubense
To study whether the immunoreactions of banana contribute the pathogenic differences between physiological races 1 (Foc1) and 4 (Foc4) of Fusarium oxysporum f. sp. cubense (Foc), we monitored the resistance react...
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Article
Open AccessGenome-wide characterization of a SRO gene family involved in response to biotic and abiotic stresses in banana (Musa spp.)
Banana (Musa spp.) is one of the world’s most important fruits and its production is largely limited by diverse stress conditions. SROs (SIMILAR TO RCD-ONE) have important functions in abiotic stress resistance a...
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Article
Open AccessDeciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soil
Fusarium wilt of banana (Musa spp.) caused by the fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) is a typical soilborne disease, that severely devastates the banana industry worldwide, and soil microbial...