25 Result(s)
within
**gao Dong
Life Sciences
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Article
Non-Rabl Patterns of Centromere and Telomere Distribution in the Interphase Nuclei of Plant Cells
At the anaphase of cell divisions, the divided chromosomes move to the two poles, with the centromeres as heads and telomeres as tails. Such a polarized orientation of centromeres and telomeres is believed to ...
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Article
Distribution of mating types and genetic diversity induced by sexual recombination in Setosphaeria turcica in northern China
Mature ascocarps and ascospores in the heterothallic ascomycete fungus, Setosphaeria turcica, were successfully produced in Sach’s medium with barley culm as the mating stimulator after four weeks’ coincubation o...
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Cloning and structure analysis of an NBS-LRR disease-resistant gene from Setaria italica Beauv
Degenerate PCR primers targeting conserved motifs of most NBS-LRR disease-resistant genes in plants were tested in Setaria italica Beauv. cultivar Shilixiang, which is resistant to Uromyces setariae-italicae. A s...
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Expression analysis of RUS1 and construction of RUS1 plant expressing vector
RUS1 was one of the disease resistance gene analogs obtained from Setaria italica Beauv. Semi-quantitative RT-PCR analysis result showed that RUS1 gene could be induced by Uromyces setariae-italicae and had relat...
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Knockdown of ACS9 expression in Arabidopsis decreases the tolerance to salt and osmotic stress
Based on the DNA sequence of ACS9, two produced fragments were subcloned into binary vector pCAMBIA1300 in antisense and sense orientations, and the generated RNA interference (RNAi) vector was then transformed i...
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Article
A proteomic approach to investigating the promotive effects of brassinolide on root growth of rice seedlings
Brassinolide influenced rice (Oryza sativa L.) root growth in a concentration-dependent manner. Roots grown in 0.1 nmol/L BL were 28% longer than those in the control. On the other hand, the roots grown in 1000 n...
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Cloning and pharmaceutical analysis of CaMK gene of Botrytis cinerea
Using a PCR homology approach, DNA and cDNA sequences of calcium/calmodulin-dependent protein kinase (CaMK) gene of Botrytis cinerea were obtained. Southern blotting result displayed that CaMK was single copy in ...
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Article
Construction of RNAi vector for flower-related gene and verifications of the mutant in Arabidopsis thaliana
The timing of floral transition is tightly controlled by a combination of endogenous and environmental signals. One early flowering mutant plant was screened from Arabidopsis library of T-DNA insertion to acceler...
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Article
BcDR1, a putative gene, regulates the development and pathogenicity of Botrytis cinerea
Botrytis cinerea is one of the important phytopathogenic fungi. Cloning of the genes related to their development and pathogenicity is fundamental to the pathogen control. A mutant (BCt16...
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Article
Identification of a gene responsible for the 60-day delay in flowering time of Arabidopsis
Identification of genes related to flowering-time in Arabidopsis is very important and meaningful contribution to the flowering process control. One late flowering mutant plant, which exhibits 60-day delay in flo...
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Article
mRNA level of PKA-c gene in Setosphaeria turcica with different nutrition sources under metal ion or osmotic stress
Intracellular signal transduction pathways including MAPK, Ca2+, and cAMP signal transduction pathways play important roles in regulating growth, development, and pathogenesis of phytopathogenic fungi. Protein ki...
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Article
Identification and molecular tagging of two Arabidopsis resistance genes to Botrytis cinerea
To map Arabidopsis resistance genes to Botrytis cinerea, Arabidopsis Col-0 ecotype resistant to B. cinerea BC18 isolate and Arabidopsis Ler ecotype susceptible to B. cinerea BC18 isolate were crossed. According t...
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Article
Screening of conidium development mutant of Botrytis cinerea and functional analysis of the related gene
A novel conidium development mutant was obtained by screening the transformants of Botrytis cinerea produced by Agrobacterium tumefaciens mediated method, which lost the ability of producing conidia. The flanking...
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Article
Occurrence of major mycotoxins in maize from Hebei Province, China
To investigate the frequency of occurrence and the concentrations of aflatoxins (AFs), deoxynivalenol (DON), zearalenone (ZEN) and fumonisins (FBs) in naturally infected maize, 25 samples of maize collected fr...
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Article
Mutations in AP22.65 accelerate flowering in Arabidopsis thaliana
Identification of the gene(s) responsible for flowering time in Arabidopsis has significant implications. We used the T-DNA insertion library of Arabidopsis thaliana to screen an early-flowering mutant that exhib...
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Article
Functional analysis of BT4 of Arabidopsis thaliana in resistance against Botrytis cinerea
Botrytis cinerea is a fungus with a necrotrophic lifestyle attacking over 200 crop hosts worldwide, resulting in significant economic losses. At present, the molecular and cellular mechanisms inv...
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Article
Molecular characterization of StpkaC2 and expression patterns of both PKA-c isoforms during the invasive growth of Setosphaeria turcica
In order to clarify the roles of different protein kinase A (PKA) isoforms during the pathogenesis, a gene coding the catalytic subunit of PKA (PKA-c) of S. turcica was predicted from the genome database. In addi...
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Article
D27E mutation of VTC1 impairs the interaction with CSN5B and enhances ascorbic acid biosynthesis and seedling growth in Arabidopsis
Our previous investigation revealed that GDP-Man pyrophosphorylase (VTC1), a vital ascorbic acid (AsA) biosynthesis enzyme, could be degraded through interaction with the photomorphogenic factor COP9 signaloso...
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Article
The ethylene response factor OsERF109 negatively affects ethylene biosynthesis and drought tolerance in rice
Drought is an important factor limiting plant development and crop production. Dissecting the factors involved in this process is the key for enhancement of plant tolerance to drought stress by genetic approac...
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Article
Identification of laccase-like multicopper oxidases from the pathogenic fungus Setosphaeria turcica and their expression pattern during growth and infection
Setosphaeria turcica (syn. Exserohilum turcicum) is the pathogenic fungus of maize (Zea mays) causing northern leaf blight, which is a major maize disease worldwide. Laccase-like multicopper oxidases (LMCOs) are ...
