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Article
Open AccessQuantitatively map** local quality of super-resolution microscopy by rolling Fourier ring correlation
In fluorescence microscopy, computational algorithms have been developed to suppress noise, enhance contrast, and even enable super-resolution (SR). However, the local quality of the images may vary on multipl...
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Article
Mitopherogenesis, a form of mitochondria-specific ectocytosis, regulates sperm mitochondrial quantity and fertility
Mitochondrial export into the extracellular space is emerging as a fundamental cellular process implicated in diverse physiological activities. Although a few studies have shed light on the process of discardi...
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Article
Open AccessMechanical instability generated by Myosin 19 contributes to mitochondria cristae architecture and OXPHOS
The folded mitochondria inner membrane-cristae is the structural foundation for oxidative phosphorylation (OXPHOS) and energy production. By mechanically simulating mitochondria morphogenesis, we speculate tha...
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Article
Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy
A main determinant of the spatial resolution of live-cell super-resolution (SR) microscopes is the maximum photon flux that can be collected. To further increase the effective resolution for a given photon flu...
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Article
Open AccessAn explainable supervised machine learning predictor of acute kidney injury after adult deceased donor liver transplantation
Early prediction of acute kidney injury (AKI) after liver transplantation (LT) facilitates timely recognition and intervention. We aimed to build a risk predictor of post-LT AKI via supervised machine learning...
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Article
Open AccesspHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis docking and fusion steps
pH-sensitive fluorescent proteins (FPs) are highly advantageous for the non-invasive monitoring of exocytosis events. Superecliptic pHluorin (SEP), a green pH-sensitive FP, has been widely used for imaging sin...
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Article
Algicidal properties of fermentation products from Bacillus cereus strain JZBC1 dissolving dominant dinoflagellate species Scrippsiella trochoidea, Prorocentrum micans, and Peridinium umbonatum
Intensive and semi-intensive aquaculture systems typically produce high loads of nutrients in their discharge, potentially leading to the development and persistence of harmful algal blooms (HABs). Algicidal b...
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Article
Open AccessOPA1 and MICOS Regulate mitochondrial crista dynamics and formation
Mitochondrial cristae are the main site for oxidative phosphorylation, which is critical for cellular energy production. Upon different physiological or pathological stresses, mitochondrial cristae undergo rem...
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Article
Open AccessSuper-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome
The emergence of super-resolution (SR) fluorescence microscopy has rejuvenated the search for new cellular sub-structures. However, SR fluorescence microscopy achieves high contrast at the expense of a holisti...
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Article
Open AccessA protocol for structured illumination microscopy with minimal reconstruction artifacts
The imaging rate of structured illumination microscopy (SIM) reached 188 Hz recently. As the exposure time decreases, the camera detects fewer virtual photons, while the noise level remains the same. As a resu...
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Article
Open AccessE-syt1 Re-arranges STIM1 Clusters to Stabilize Ring-shaped ER-PM Contact Sites and Accelerate Ca2+ Store Replenishment
In many non-excitable cells, the depletion of endoplasmic reticulum (ER) Ca2+ stores leads to the dynamic formation of membrane contact sites (MCSs) between the ER and the plasma membrane (PM), which activates th...
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Article
Microvascular endothelial cells engulf myelin debris and promote macrophage recruitment and fibrosis after neural injury
The clearance of damaged myelin sheaths is critical to ensure functional recovery from neural injury. Here we show a previously unidentified role for microvessels and their lining endothelial cells in engulfin...
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Chapter and Conference Paper
Using Electrically Tunable Lens to Improve Axial Resolution and Imaging Field in Light Sheet Fluorescence Microscope
Light sheet fluorescence microscope (LSFM) has become one of the most promising 3D microscopy techniques due to its prominent lateral section illumination. In addition, comparing with the confocal microscope, ...
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Article
Fast, long-term, super-resolution imaging with Hessian structured illumination microscopy
An improved image reconstruction algorithm increases time resolution and maximal imaging time for super-resolution microscopy.
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Article
Open AccessZyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules
Endothelial exocytosis of Weibel–Palade body (WPB) is one of the first lines of defence against vascular injury. However, the mechanisms that control WPB exocytosis in the final stages (including the docking, ...
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Article
Structure and dielectric properties of Nd substituted Bi1.5MgNb1.5O7 ceramics
New pyrochlore ceramics Bi(1.5−x)Nd x MgNb1.5O7 with x = 0–0.6 were synthesized by do** Nd into the A site in the Bi1.5MgNb1.5O7 pyrochlore. The Nd substituted Bi1.5MgNb1.5O7 c...
