Search
Search Results
-
Imaging Cell Signaling in Tissues Using the IBEX Method
The study of cell signaling within tissues can be enhanced using highly multiplexed immunohistochemistry to localize the presence and spatial...
-
In Situ Imaging of Proteins Using DNA-PAINT Super-Resolution Microscopy
The spatial resolution of conventional light microscopy is restricted by the diffraction limit to hundreds of nanometers. Super-resolution microscopy...
-
Deep Learning-Based Cell Tracking in Deforming Organs and Moving Animals
Cell tracking is an essential step in extracting cellular signals from moving cells, which is vital for understanding the mechanisms underlying...
-
Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue
Macrophages are one of the prominent leukocyte populations in white adipose tissue (WAT) and play an important role during WAT homeostasis and...
-
Combined Host-Pathogen Fate Map** to Investigate Lung Macrophages in Viral Infection
Macrophage identity, as defined by epigenetic, transcriptional, proteomic, and functional programs, is greatly impacted by cues originating from the...
-
Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation
InflammasomesInflammasomes are macromolecular complexes that assemble upon the detection of cytoplasmic pathogen-associated or danger-associated...
-
Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy
Innately present in tears, saliva and mucosal secretions, lysozyme provides a critical defensive strategy to the host by cleaving the...
-
A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening
To improve our understanding of human placental function and placental cell responses to pregnancy stressors, the development of in vitro models that...
-
Precision-Cut Slice Culture Method for Rat Placenta
Organotypic slice cultures provide several advantages over primary and cell line culture models. Unlike monolayer cultures, organotypic slice...
-
Three-Dimensional In Vitro Human Placental Organoids from Mononuclear Villous Trophoblasts or Trophoblast Stem Cells to Understand Trophoblast Dysfunction in Fetal Growth Restriction
The placentaPlacenta plays a critical role in the efficient delivery of nutrients and oxygen from mother to fetus to maintain normal fetal growth....
-
Expansion Microscopy of Ciliary Proteins
Label-retention expansion microscopy (LR-ExM) is a sample preparation technique, which embeds the cells or tissues in a swellable hydrogel and...
-
Structural Analysis of Sperm Centrioles Using N-STORM
A prominent technical barrier when imaging swimming sperm is capturing a singular sperm cell’s head and tail position simultaneously at a high...
-
A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium
Volume electron microscopy technologies such as serial block face scanning electron microscopy (SBF-SEM) allow the characterization of tissue...
-
Computational Modeling and Simulation of the Fibrotic Human Atria
Patient-specific modeling of atrial electrical activity enables the execution of simulations that can provide mechanistic insights and provide novel...
-
Whole-Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosis
Whole-mount immunofluorescence allows direct visualization of the cellular architecture within cells. Here, we apply this technique to mouse oocytes...
-
Develo** a Prospective Gestational Lyme Disease Study
Lyme disease in pregnancy is understudied. The few available reports of Borrelia infection during pregnancy collecting clinical outcomes, with or...
-
Identifying Transmembrane Interactions in Receptor Protein Tyrosine Phosphatase Homodimerization and Heterodimerization
Receptor protein tyrosine phosphatases (RPTPs) are one of the key regulators of receptor tyrosine kinases (RTKs) and therefore play a critical role...
-
Alpha-1-Antitrypsin (A1AT) Proteoty** by LC-MS/MS
The diagnosis of alpha-1-antitrypsin (A1AT) deficiency is established by quantitation of protein concentration in serum (immunoassay) followed by...
-
Isolation, Culture, and Microscopic Imaging of Guinea Pig Primary Gastric Tissue Cells
In this chapter, the procedure of isolation and propagation of guinea pig gastric tissue primary cells in cell culture in vitro is presented....
-
Manipulation of Proteostasis Networks in Transgenic ZAAT Zebrafish via CRISPR–Cas9 Gene Editing
The CRISPR–Cas9 genome editing system is used to induce mutations in genes of interest resulting in the loss of functional protein. A transgenic...