Abstract
Inflammasomes are macromolecular complexes that assemble upon the detection of cytoplasmic pathogen-associated or danger-associated signals and induce a necrotic type of cell death termed pyroptosis, facilitating pro-inflammatory cytokine release. Inflammasomes play a critical role in innate immunity and inflammatory response; however, they have also been associated with multiple diseases, including autoinflammatory and neurodegenerative conditions. In the following chapter, we describe methods to detect inflammasome activation and its downstream effects, including detection of ASC oligomerization, detection of activated caspase-1 and cleaved IL-1β, as well as read-outs for inflammasome-mediated cell death.
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Acknowledgments
All schematics were created with BioRender. K.S. is a recipient of SNSF Postdoc. Mobility fellowship (P500PB_211096). R.M. is supported by the Alzheimer Forschung Initiative (#20043). E.L. is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy (EXC2151—390873048) and Helmholtz Association, under the project title “Immunology&Inflammation,” (#ZT-0027).
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Lovotti, M., Mangan, M.S.J., McManus, R.M., Shkarina, K., Vasconcelos, M.B., Latz, E. (2024). Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation. In: Mass, E. (eds) Tissue-Resident Macrophages. Methods in Molecular Biology, vol 2713. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3437-0_29
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DOI: https://doi.org/10.1007/978-1-0716-3437-0_29
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