7 Result(s)
within
Protocol
Zhen-** Zhang
English
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Protocol
Systems Biology: Identifiability Analysis and Parameter Identification via Systems-Biology-Informed Neural Networks
The dynamics of systems biological processes are usually modeled by a system of ordinary differential equations (ODEs) with many unknown parameters that need to be inferred from noisy and sparse measurements. ...
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Protocol
PAL-Mediated Ligation for Protein and Cell-Surface Modification
Peptidyl Asx-specific ligases (PALs) effect peptide ligation by catalyzing transpeptidation reactions at Asn/Asp-peptide bonds. Owing to their high efficiency and mild aqueous reaction conditions, these ligase...
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Protocol
Detection of Uveal Melanoma by Multiplex Immunoassays of Serum Biomarkers
Multiplex immunoassays simultaneously measure multiple analytes in a single sample providing quantitative data via parallel analyses, which is especially suitable for serum biomarker verification and validatio...
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Protocol
Development of Transgenic CryIA(c) + GNA Cotton Plants via Pollen-Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover
Elite cotton cultivar Sumian16 was transformed with p7RPSBK-mGNA-NPTII containing Bt (CryIA(c)), Galanthus nivalis agglutinin (GNA) resistance genes, and selectable marker NptII gene via the pollen-tube pathway m...
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Protocol
Synthesis and Evaluation of Novel Neamine–Nucleoside Conjugates as Potential Antibiotic Targets for Escherichia coli 16S Ribosomal RNA
Based on the nucleobase rich character of the binding pocket of A-site 16S ribosomal RNA of Escherichia coli, it was proposed that the neamine moiety of synthesized Neamine–nucleoside conjugates could bind to the...
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Protocol
Using the CPTAC Assay Portal to Identify and Implement Highly Characterized Targeted Proteomics Assays
The Clinical Proteomic Tumor Analysis Consortium (CPTAC) of the National Cancer Institute (NCI) has launched an Assay Portal (http://assays.cancer.gov) to serve as...
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Protocol
Efficient Analysis Methods in Synthetic Biology
This chapter describes new analysis and verification techniques for synthetic genetic circuits. In particular, it applies stochastic model checking techniques to models of genetic circuits in order to ensure that...
