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Protocol
Ethanol Injection Method for Liposome Preparation
Ethanol injection method is one of the preferred methods for liposome preparation due to its advantages including rapidity, safety, and reproducibility. This method involves the injection of phospholipid solut...
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Protocol
Genoty** of Transposable Element Insertions Segregating in Human Populations Using Short-Read Realignments
Transposable element (TE) insertions are a major source of in the human genome. Due to the repetitive nature and biological importance of TEs, many tools have been developed to identify and genotype TE ins...
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Protocol
CRISPR-Cas9 Gene Editing to Generate Isoform-Specific LAMP-2A Knockout in Human Cancer Cells
Chaperone-mediated autophagy (CMA) is a highly specific lysosomal-dependent protein degradation pathway. A critical molecular component of CMA is the lysosome-associated membrane protein (LAMP) type 2A, which ...
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Protocol
Tissue-Specific Ribosome Profiling in Drosophila
Robust mechanisms exist that serve to dynamically regulate the translation of mRNA into proteins across heterogeneous tissues. These processes ensure timely generation of proteins in quantities that scale with...
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Protocol
Desiccation Tolerance Assays for Acinetobacter baumannii
Acinetobacter baumannii is a hospital-associated pathogen of growing importance and is a paradigm for endemic hospital contamination. Desiccation tolerance has been implicated as an important characteristic that ...
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Protocol
Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method
Site-directed mutagenesis has been widely used for the substitution, addition or deletion of nucleotide residues in a defined DNA sequence. QuikChange™ site-directed mutagenesis and its related protocols have ...
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Protocol
Rational Design of Rho GTPase-Targeting Inhibitors
Rho GTPases have been implicated in diverse cellular functions and are potential therapeutic targets in inflammation, cancer, and neurologic diseases. Virtual screening of compounds that fit into surface groov...
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Protocol
Using ChIPMotifs for De Novo Motif Discovery of OCT4 and ZNF263 Based on ChIP-Based High-Throughput Experiments
DNA motifs are short sequences varying from 6 to 25 bp and can be highly variable and degenerated. One major approach for predicting transcription factor (TF) binding is using position weight matrix (PWM) to r...
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Protocol
Rho GTPases in Hematopoietic Stem/Progenitor Cell Migration
Rho GTPases including RhoA, Rac1, and Cdc42 are a class of intracellular signaling proteins critical for the regulation of cytoskeleton organization, adhesion, and migration. Molecular mechanisms of mammalian ...
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Protocol
Fractionation of Complex Proteomes by Microscale Solution Isoelectrofocusing Using ZOOM− IEF Fractionators to Improve Protein Profiling
All current methods for quantitatively comparing protein profiles, including twodimensional polyacrylamide gel electrophoresis (2-D PAGE) as well as non-2-D gel methods such as isotope-coded affinity tag metho...
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Protocol
Microscale Solution Isoelectrofocusing
Proteomics is a relatively new research discipline that deals with systematic, large-scale analyses of proteins in biological systems (1,2). Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is curren...
