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    Protocol

    Continual Conscious Bioluminescent Imaging in Freely Moving Mice

    In vivo bioluminescent imaging allows the detection of reporter gene expression in rodents in real time. Here we describe a novel technology whereby we can generate somatotransgenic rodents with the use of a v...

    Juan Antinao Diaz, Amy Geard, Lorna M. FitzPatrick… in Bioluminescent Imaging (2020)

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    Protocol

    Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivo

    The application of luciferase reporter to provide quantitative outputs for the activation of promoters is a well-established technique in molecular biology. Luciferase catalyzes an enzymatic reaction, which ...

    Juliette M. K. M. Delhove, Rajvinder Karda… in Mammalian Synthetic Promoters (2017)