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  1. No Access

    Protocol

    Blue Native PAGE Analysis of Bacterial Secretion Complexes

    Bacterial protein secretion systems serve to translocate substrate proteins across up to three biological membranes, a task accomplished by hydrophobic, membrane-spanning macromolecular complexes. The overexpr...

    Susann Zilkenat, Eun** Kim, Tobias Dietsche… in Bacterial Secretion Systems (2024)

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    Protocol

    Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay

    Studies of bacterial protein secretion have relied on a variety of reporters that allow the tracking of secreted proteins. However, the lack of truly quantitative and highly sensitive reporters has hindered, i...

    Sara Vilela Pais, Sibel Westerhausen, Erwin Bohn, Samuel Wagner in Bacterial Virulence (2022)

  3. No Access

    Protocol

    Blue Native PAGE Analysis of Bacterial Secretion Complexes

    Bacterial protein secretion systems serve to translocate substrate proteins across up to three biological membranes, a task accomplished by hydrophobic, membrane-spanning macromolecular complexes. The overexpr...

    Susann Zilkenat, Tobias Dietsche… in Bacterial Protein Secretion Systems (2017)

  4. No Access

    Protocol

    Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-PAGE

    Two-dimensional blue native (2D BN)/SDS-PAGE is the method of choice for the global analysis of the subunits of complexes in membrane proteomes. In the 1st dimension complexes are separated by BN-PAGE, and in ...

    Susan Schlegel, Mirjam Klepsch, David Wickström, Samuel Wagner… in Protein Secretion (2010)