6 Result(s)
within
Protocol
Ryohei Yasuda
-
Protocol
FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs
With the development of fluorescent proteins (FPs) and advanced optical microscopy techniques, Förster or fluorescence resonance energy transfer (FRET) has become a powerful tool for real-time noninvasive visu...
-
Protocol
Correlative Ultrastructural Analysis of Functionally Modulated Synapses Using Automated Tape-Collecting Ultramicrotome and SEM Array Tomography
Live imaging of dendritic spines using advanced light microscopy (LM) provides insight into how the brain processes information to learn and form memories. As a complementary approach, electron microscopy (EM)...
-
Protocol
Analysis of TrkB Receptor Activity Using FRET Sensors
Here, we describe the use of 2-photon fluorescence lifetime imaging (2pFLIM) of a Förster resonance energy transfer (FRET) sensor to study the spatial and temporal activity pattern of the BDNF receptor TrkB. C...
-
Protocol
Imaging Neuronal Signal Transduction Using Multiphoton FRET-FLIM
Synaptic plasticity, the ability of neurons to modulate the strength of specific inputs, is critical for neural circuits to adapt to experience throughout life. In excitatory pyramidal neurons, plasticity is i...
-
Protocol
Imaging Signaling Transduction in Single Dendritic Spines
Synaptic plasticity, like many cellular functions, is an extremely complex process that requires a vast network of signaling elements comprising hundreds of protein species. As synaptic plasticity is caused by...
-
Protocol
Imaging the Activity of Ras Superfamily GTPase Proteins in Small Subcellular Compartments in Neurons
Resolving the spatiotemporal dynamics of intracellular signaling is important for understanding the molecular mechanisms of various cellular processes induced by extracellular signals. Two-photon fluorescence ...
