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Protocol
Selecting Channelrhodopsin Constructs for Optimal Visual Restoration in Differing Light Conditions
Channelrhodopsin (ChR)-based optogenetics is one promising approach to restore vision in photoreceptor degenerative diseases such as retinitis pigmentosa (RP) and age-related macular degeneration (AMD). Curren...
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Protocol
LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence
Calpains are a family of Ca2+-dependent cysteine proteases involved in many important biological processes, where they selectively cleave relevant substrates at specific cleavage sites to regulate the function of...
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Protocol
Metaproteomics Study of the Gut Microbiome
Proteomics is a widely used method for defining the protein composition of a complex sample. As this approach allows for identification and quantification of proteins across a broad dynamic range as well as de...
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Protocol
Quantitative Glycoproteomics for N-Glycoproteome Profiling
Glycoproteomics, the global analysis of glycoproteins, is a subfield of proteomics with high biological and clinical relevance. Glycoproteomics integrates knowledge and techniques drawing from glycotechnology,...
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Protocol
Nanofluidic Devices for Rapid Continuous-Flow Bioseparation
Compared with conventional gel-based techniques, such as gel electrophoresis, which are routinely used for bioseparation in biology and biomedical laboratories, nanofluidic devices with regular engineered siev...
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Protocol
Proteomic Analysis of Caenorhabditis elegans
Proteomic studies of the free-living nematode Caenorhabditis elegans have recently received great attention because this animal model is a useful platform for the in vivo study of various biological problems rele...