14 Result(s)
within
English
Life Sciences
Biochemistry, general
Pan **g
Protocol
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Protocol
Amino Acid Analysis With Postcolumn Fluorescent Derivatization
Amino acid analysis may be considered to be a fundamental technique for all aspects of protein chemistry. It is used not only for obtaining new data, but also for confirming the results from other types of ana...
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Quantitative Proteomics by Stable Isotope Labeling and Mass Spectrometry
The goal of quantitative proteomics is to systematically study static state or perturbation-induced changes in protein profile. Most of the recently developed mass spectrometry (MS)-based quantitative proteomi...
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Visualization of Syntenic Relationships With SynBrowse
Synteny is the preserved order of genes between related species. To detect syntenic regions one usually first applies sequence comparison methods to the genomic sequences of the considered species. Sequence si...
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Analysis of Genetic Interactions on a Genome-Wide Scale in Budding Yeast: Diploid-Based Synthetic Lethality Analysis by Microarray
Comprehensive collections of open reading frame (ORF) deletion mutant strains exist for the budding yeast Saccharomyces cerevisiae. With great prescience, these strains were designed with short molecular bar code...
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In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells
Adult bone marrow stem cell is an ideal target for gene therapy of genetic diseases, selected malignant diseases, and AIDS. The in vivo approach of lentivirus vector (LV)-mediated stem cell gene transfer by in...
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Proteomic Analysis of Caenorhabditis elegans
Proteomic studies of the free-living nematode Caenorhabditis elegans have recently received great attention because this animal model is a useful platform for the in vivo study of various biological problems rele...
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Primer-Free Aptamer Selection Using a Random DNA Library
Aptamers are high-affinity oligonucleotides which can be selected from large random libraries by systematic evolution of ligands by exponential enrichment (SELEX) protocols, with affinities and specificities c...
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Monitoring MicroRNA Expression During Embryonic Stem-Cell Differentiation Using Quantitative Real-Time PCR (qRT-PCR)
Quantitative real-time PCR (qRT-PCR) is a reliable method to determine and monitor microRNA (miRNA) expression profiles in different cells, tissues, and organisms. Although there are several different strategi...
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Generation of a Proteolytic Signal: E3/E2-Mediated Polyubiquitination of IκBα
A key regulatory node in NF-κB signaling is the removal of the IκBα inhibitor, whose levels are tightly controlled by the ubiquitin–proteasome system. In response to signal activation and transmission, ubiquit...
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Protein–Protein Interaction Inhibitors of BRCA1 Discovered Using Small Molecule Microarrays
Microarray screening technology has transformed the life sciences arena over the last decade. The platform is widely used in the area of map** interaction networks, to molecular fingerprinting and small mole...
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Transfer Zymography
The technique described here, transfer zymography, was developed to overcome two limitations of conventional zymography. When proteolytic enzymes are resolved by nonreducing SDS-PAGE into a polyacrylamide gel ...
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Mn2+ Quenching Assay for Store-Operated Calcium Entry
Store-operated Ca2+ entry (SOCE) pathway plays important roles in many cellular processes, which is largely studied by using fluorescent Ca2+ indicator, Fura-2. Extracellular Mn2+ is able to cross the plasma memb...
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Develo** High-Throughput Assays to Analyze and Screen Electrophysiological Phenotypes
Ion channels represent nearly a quarter of all targets that currently available medications modulate, and their dysfunction underlies increasing number of human diseases. Functional analysis of ion channels ha...
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Measurement of Intracellular Ca2+ for Studying GPCR-Mediated Lipid Signaling
Intracellular Ca2+ can be conveniently monitored by sensitive Ca2+ fluorescent dyes in live cells. The Gαq involved lipid signaling pathways and, thus, can be studied by intracellular Ca2+ imaging. Here we descri...
