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Protocol
In Silico Design of Recombinant Chimera T Cell Peptide Epitope Vaccines for Visceral Leishmaniasis
Visceral leishmaniasis (VL) is a neglected tropical disease caused by protozoan parasites of the genus Leishmania. Systemic VL is fatal if untreated and there are no prophylactic human vaccines available. Several...
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Protocol
Preclinical Assessment of the Immunogenicity of Experimental Leishmania Vaccines
Leishmaniases are neglected diseases caused by Leishmania parasites and affect millions of people worldwide. The induction of protective immunity against infection by some species of Leishmania has stimulated the...
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Protocol
Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae
Neisseria gonorrhoeae is a gram-negative obligate human pathogen that contains lipooligosaccharide (LOS) as a major constituent within the outer membrane. LOS plays a major role in pathogenesis by inducing host i...
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Protocol
Basic Methods for Examining Neisseria gonorrhoeae Interactions with Host Cells In Vitro
The obligate human pathogen Neisseria gonorrhoeae colonizes primarily the mucosal columnar epithelium of the male urethra and the female endocervix. In addition, gonococci can infect the anorectal, pharyngeal, an...
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Protocol
Use of Human Fallopian Tube Organ in Culture (FTOC) and Primary Fallopian Tube Epithelial Cells (FTEC) to Study the Biology of Neisseria gonorrhoeae Infection
Epithelial cells represent one of the most important physical barriers to many bacterial pathogens. In the case of Neisseria gonorrhoeae, the epithelial cell response is critical because they are the main target ...
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Protocol
A DNA Vaccine Strategy for Effective Antibody Induction to Pathogen-Derived Antigens
DNA-based vaccines are currently being developed for treating a diversity of human diseases including cancers, autoimmune conditions, allergies, and microbial infections. In this chapter, we present a general ...
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Protocol
Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach
The availability of Neisseria genome sequences together with improvements in proteomic technologies provide the opportunity to study at high resolution the immune response to Neisseria meningitidis. In this chapt...
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Protocol
Recombinant Proteins in Vaccine Development
The outer membrane of Neisseria meningitidis contains a variety of proteins with the potential for inclusion in new meningococcal vaccines (1). Studies on the vaccine potential of these proteins would be facilita...
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Protocol
Epitope Map**
An epitope is defined as the site on an antigen at which an antibody binds. In the case of proteins the epitopes can be classified as continuous (or sequential) and discontinuous according to whether or not th...