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Protocol
Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos
Amphibian oocytes and embryos are classical models to study cellular and developmental processes. For these studies, it is often advantageous to visualize protein organization. However, the large size and yolk...
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Protocol
Quantitative Proteomics for Xenopus Embryos II, Data Analysis
The oocytes, embryos, and cell-free lysates of the frog Xenopus laevis have emerged as powerful models for quantitative proteomic experiments. In the accompanying paper (Chapter 13) we describe how to prepare sam...
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Protocol
Quantitative Proteomics of Xenopus Embryos I, Sample Preparation
Xenopus oocytes and embryos are model systems optimally suited for quantitative proteomics. This is due to the availability of large amount of protein material and the ease of physical manipulation. Furthermore, ...