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    Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos

    Amphibian oocytes and embryos are classical models to study cellular and developmental processes. For these studies, it is often advantageous to visualize protein organization. However, the large size and yolk...

    Thao Nguyen, Timothy J. Mitchison, Martin Wühr in Vertebrate Embryogenesis (2019)

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    Quantitative Proteomics for Xenopus Embryos II, Data Analysis

    The oocytes, embryos, and cell-free lysates of the frog Xenopus laevis have emerged as powerful models for quantitative proteomic experiments. In the accompanying paper (Chapter 13) we describe how to prepare sam...

    Matthew Sonnett, Meera Gupta, Thao Nguyen, Martin Wühr in Xenopus (2018)

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    Quantitative Proteomics of Xenopus Embryos I, Sample Preparation

    Xenopus oocytes and embryos are model systems optimally suited for quantitative proteomics. This is due to the availability of large amount of protein material and the ease of physical manipulation. Furthermore, ...

    Meera Gupta, Matthew Sonnett, Lillia Ryazanova, Marc Presler, Martin Wühr in Xenopus (2018)