7 Result(s)
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Protocol
Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies
The activation of ATM is critical in the DNA double strand breaks repair pathway. Acetylation of ATM by Tip60 histone acetyltransferase (HAT) plays a key role in the activation of ATM kinase activity in respon...
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Protocol
Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus
Porcine reproductive and respiratory syndrome (PRRS) is a reemerging swine disease, and has become economically the most significant disease in pork production worldwide. The causative agent is PRRS virus (PRR...
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Protocol
In Silico Prediction of RNA Secondary Structure
The secondary structure of an RNA molecule represents the base-pairing interactions within the molecule and fundamentally determines its overall structure. In this chapter, we overview the main approaches and ...
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Protocol
Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System
Long-term hematopoietic stem cells (LT-HSCs) have the ability to self-renew and differentiate into all blood cell lineages. Understanding the genetic networks that regulate LT-HSC function in the adult bone ma...
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Protocol
miRNA and shRNA Expression Vectors Based on mRNA and miRNA Processing
RNA interference (RNAi) has emerged as a powerful tool in basic research and therapeutics by silencing the expression of specific target genes. RNAi is triggered by double-stranded small interfering RNAs, whic...
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Protocol
Monitoring I-SceI-Induced Double-Strand Break Repair in DT40 Cells
The chicken B lymphocyte cell line DT40 is now a well-established system for studying DNA repair in vertebrates. Central to its utility is its ready genetic tractability allowing for the creation of isogenic m...
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Protocol
ENU Mutagenesis in Mice
Forward genetics has led to many “breakthrough” discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by wh...
