14 Result(s)
within
Protocol
Kirankumar S. Mysore
-
Protocol
Virus-Induced Gene Silencing in Sorghum Using Brome Mosaic Virus
Sorghum [Sorghum bicolor (L.) Moench.] is a versatile crop, grown in 30 countries and a food source for nearly 500 million people globally. Although the sorghum genome is sequenced, a limited understanding of gen...
-
Protocol
High-Throughput Analysis of Gene Function under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants
The high throughputness and affordability of “omics” technologies is leading to the identification of a large number of abiotic stress genes, with many of them responsive to multiple stresses. In vivo function...
-
Protocol
Recent Advances in Plant Gene Silencing Methods
With the increasing understanding of fundamentals of gene silencing pathways in plants, various tools and techniques for downregulating the expression of a target gene have been developed across multiple plant...
-
Protocol
Tnt1 Insertional Mutagenesis in Medicago truncatula
Legumes play irreplaceable roles in sustainable agriculture due to their unique capability of fixing gaseous nitrogen in the atmosphere and turning into plant-usable ammonium through interaction with rhizobia....
-
Protocol
Tissue Culture (Somatic Embryogenesis)-Induced Tnt1 Retrotransposon-Based Mutagenesis in Brachypodium distachyon
Brachypodium distachyon is a model grass species for economically important cereal crops. Efforts are in progress to develop useful functional genomic resources in Brachypodium. A tobacco retrotransposon, Tnt1, ...
-
Protocol
Enabling Reverse Genetics in Medicago truncatula Using High-Throughput Sequencing for Tnt1 Flanking Sequence Recovery
The genome sequence of Medicago truncatula was published and released in 2011. A Tnt1 insertional mutant population with 21,700 independently regenerated lines was completed in 2012 at The Samuel Roberts Noble Fo...
-
Protocol
Advances in Plant Gene Silencing Methods
Understanding molecular mechanisms of transcriptional and posttranscriptional gene silencing pathways in plants over the past decades has led to development of tools and methods for silencing a target gene in ...
-
Protocol
Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines
A large population of Medicago truncatula insertion lines has been generated using the Tnt1 retrotransposon. More than 21,000 insertion lines have been generated, representing more than 500,000 insertion events. ...
-
Protocol
Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses
Agroinoculation, first developed as a simple tool to study plant–virus interactions, is a popular method of choice for functional gene analysis of viral genomes. With the explosive growth of genomic informatio...
-
Protocol
Caveat of RNAi in Plants: The Off-Target Effect
RNA interference (RNAi), mediated by short interfering RNAs (siRNAs), is one of the widely used functional genomics method for suppressing the gene expression in plants. Initially, gene silencing by RNAi mecha...
-
Protocol
Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants
Medicago truncatula has been chosen as one of the two model species for legume molecular genetics and functional genomics studies. With the imminent completion of M. truncatula genome sequencing, a...
-
Protocol
Functional Characterization of Water-Deficit Stress Responsive Genes Using RNAi
In response to water-deficit stress, plants alter expression of thousands of genes and as a result, cellular, physiological, and biochemical processes are modified. Understanding the functional role of water-d...
-
Protocol
Agroinoculation: A Simple Procedure for Systemic Infection of Plants with Viruses
Plant-virus interaction studies, for long, plagued by asynchronous/failed infections, have improved since the usage of Agrobacterium as a delivery agent for viral genomes. Popularly known as “agroinoculation,” th...
-
Protocol
Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOF MS Analysis
A method has been developed for the extraction of intact proteins from SDS-PAGE gels and for subsequent MALDI-TOF MS analysis to determine precise molecular mass. The method consists of an electroelution step ...
