84 Result(s)
-
Protocol
The Photothrombotic Model of Ischemic Stroke
Stroke is a major cause of morbidity worldwide; yet, there is a lack of treatment options to address post-stroke cognitive and motor impairment, thus there is an urgency for develo** neuroprotective and rest...
-
Protocol
Generation of Human Induced Pluripotent Stem Cells Using Episomal Vectors
Human induced pluripotent stem cells (iPSCs) can be reprogrammed from differentiated somatic cells, such as adult fibroblasts and peripheral blood mononuclear cells, by introducing sets of defined factors. iPS...
-
Protocol
Gene Editing and Reprogramming of Human Fibroblast Cells to Human Induced Pluripotent Stem Cells
Predictive disease models play significant roles in advancing our knowledge of the pathology of human disease. In this field, animal models have been extensively employed and have provided crucial insights int...
-
Protocol
Silicone Oil-Induced Ocular Hypertension Glaucoma Model (SOHU) in Rodent and Nonhuman Primate
In this chapter, we describe a clinically relevant inducible and reversible ocular hypertension glaucoma model, which mimics the secondary glaucoma that can be a postoperative complication when silicone oil (S...
-
Protocol
Generation of Cerebral Cortical Neurons from Human Pluripotent Stem Cells in 3D Culture
Human forebrain cortical neurons are essential for fundamental functions like memory and consciousness. Generation of cortical neurons from human pluripotent stem cells provides a great source for creating mod...
-
Protocol
Evaluation of Retinal Ganglion Cell via Visual Evoked Potential
Visual Evoked Potential (VEP) is an electrical signal recorded from the visual cortex in response to light stimulation. It can be used as an in vivo method to objectively access the functional integrity of the...
-
Protocol
Cell Dissociation of Tissues for Single-Cell Analysis in African Killifish
The single-cell RNA sequencing (scRNA-seq) is a powerful tool to characterize cell heterogeneity, trace cell lineages, and identify novel cell markers in development, regeneration, and diseases. Although disti...
-
Protocol
In Vivo Evaluation of BBB Integrity in the Post-stroke Brain
The blood–brain barrier (BBB) is a dynamic interface responsible for maintaining central nervous system (CNS) homeostasis. An intact BBB protects the brain from undesired compounds and proteins from the blood;...
-
Protocol
Multiplexed Quantitative Proteomics Analysis of Develo** Human Brain Organoids
Develo** cellular models to study progressive disorders like Alzheimer’s is a challenge due to the unknown initiator of the disease and its slow progression. Recent developments in stem cell technology have ...
-
Protocol
Quality Control for Adeno-Associated Viral Vector Production
Adeno-associated viral vectors (AAV) are frequently used by neuroscientists to deliver tools, such as biosensors and optogenetic and chemogenetic actuators, in vivo. Despite its widespread use, AAV vector char...
-
Protocol
High-Speed Neural Imaging with Synaptic Resolution: Bessel Focus Scanning Two-Photon Microscopy and Optical-Sectioning Widefield Microscopy
Brain is composed of complex networks of neurons that work in concert to underlie the animal’s cognition and behavior. Neurons communicate via structures called synapses, which typically require submicron spat...
-
Protocol
In Vivo Manipulations to Correct for Behavioral Phenotypes in Cognitive Disorder Mouse Models
Identification of human mutations associated with syndromic and non-syndromic cognitive disorders (CD) has led to the creation of several hundreds of CD mouse lines that are now available to be tested in phys...
-
Protocol
Neurogenetic Variant Analysis by Optical Genome Map** for Structural Variation Detection-Balanced Genomic Rearrangements, Copy Number Variants, and Repeat Expansions/Contractions
Structural variants in the human genome have been associated with several neurological disorders. Syndromic neurodevelopmental delay has been reported to be caused by recurrent copy number variants (CNVs) at s...
-
Protocol
Genetically Encoded Biosensors Reveal Spatiotemporal Dynamics and Cellular Heterogeneity of Neuronal Cells
Neuronal networks are among the most fascinating, yet complex, systems within biology. From neurotransmitter and neuromodulator signaling to intracellular changes in second messenger levels and enzymatic activ...
-
Protocol
Aptamer Detection of Neurodegenerative Disease Biomarkers
Neurodegenerative disease is a kind of disease caused by the degeneration of neurons and myelin sheaths. With the aging of the global population, the increase of the incidence of neurodegenerative diseases, su...
-
Protocol
Positron Emission Tomography (PET) Imaging of Metabotropic Glutamate Receptor Subtype 2 (mGlu2) Based on a Negative Allosteric Modulator Radioligand
Metabotropic glutamate receptor subtype 2 (mGlu2) is a G-protein-coupled receptor and involved in the physiopathology of several major mental disorders and neurodegenerative diseases. Nowadays, with the developme...
-
Protocol
Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules
Astrocytes play important roles in neurodevelopment and diseases. Previous studies described ways to derive astrocytes from somatic cells by going through iPSC or iNSC/iNPC intermediates. Here we describe a me...
-
Protocol
Mouse Enteric Neuronal Cell Culture
In the enteric nervous system, there exist a huge number of local intrinsic neurons, which control the gastrointestinal functions. Culture of enteric neurons provides a good model system for physiological, ele...
-
Protocol
Self-Assembling Peptide Nanofibrous Scaffolds in Central Nervous System Lesions
Central nervous system (CNS) lesion leads to the loss of tissues and functional defect. After injury, the local microenvironment typically contains potential inhibitory molecules and glial scars, which inhibit...
-
Protocol
Shared-Control Robots
This chapter reviews shared-control robots, a class of robotic device in which the surgeon and the robot simultaneously manipulate the surgical tool together. The shared-control approach seeks to exploit the s...
