470 Result(s)
within
Protocol
Ke** Zhang
Biomedicine
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Protocol
TCID50 Assay: A Simple Method to Determine Baculovirus Titer
There are many methods that can be used to determine the infectious titer of your baculovirus stock. The TCID50 method is a simple end-point dilution method that determines the amount of baculovirus virus needed ...
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Protocol
Production of Secreted Antibody in Baculovirus Expression Vector System
Monoclonal antibodies have widespread applications in disease treatment and antigen detection. They are traditionally produced using mammalian cell expression system, which is not able to satisfy the increasin...
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Protocol
The Photothrombotic Model of Ischemic Stroke
Stroke is a major cause of morbidity worldwide; yet, there is a lack of treatment options to address post-stroke cognitive and motor impairment, thus there is an urgency for develo** neuroprotective and rest...
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Protocol
Split-Luciferase Complementation Imaging Assay in Virus–Plant Interactions
Protein−protein interactions constitute the interface between a virus and the cell it infects and are crucial determinants of the outcome of the viral infection. Multiple techniques have been developed to stud...
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Protocol
Generation of Human Induced Pluripotent Stem Cells Using Episomal Vectors
Human induced pluripotent stem cells (iPSCs) can be reprogrammed from differentiated somatic cells, such as adult fibroblasts and peripheral blood mononuclear cells, by introducing sets of defined factors. iPS...
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In Silico: Predicting Intrinsic Features of HLA Class-I Restricted Neoantigens
Mutation-containing immunogenic peptides from tumor cells, also named as neoantigens, have various amino acid descriptors and physical-chemical properties characterized intrinsic features, which are useful in ...
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Protocol
Profiling Numerical and Structural Chromosomal Instability in Different Cancer Types
Many cancers display whole chromosome instability (W-CIN) and structural chromosomal instability (S-CIN), referring to increased rates of acquiring numerically and structurally abnormal chromosome changes. Thi...
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Protocol
N-Glycan Insertion for Probing Protein–Protein Interactions and Epitope Map**
Introducing an N-linked glycosylation motif into recombinant proteins at specific sites is a useful tool in probing protein–protein interactions and epitope map**. Due to their large size, a new N-glycan can...
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Protocol
Designing High Binding Affinity Peptides for MHC Class I Using MAM: An In Silico Approach
The availability of extensive MHC-peptide binding data has boosted machine learning-based approaches for predicting binding affinity and identifying binding motifs. These computational tools leverage the wealt...
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Protocol
Wolbachia Transinfection Via Embryonic Microinjection
The process of transferring Wolbachia from one species to another to establish a stable, maternally inherited infection in the target species is known as transinfection. The success of transinfection is primarily...
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Protocol
Evaluation of Oxygen Consumption Rates In Situ
An analysis of the mitochondrial respiration function represented by the oxygen consumption rate is necessary to assess mitochondrial bioenergetics and redox function. This protocol describes two alternative t...
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Protocol
Studying the Dynamics of Tunneling Tubes and Cellular Spheres
Cancer cytogenetic analyses often involve cell culture. However, many cytogeneticists overlook interesting phenotypes associated with cultured cells. Given that cytogeneticists need to focus more on phenotypes...
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Protocol
A Robust In Vitro Co-culture Model for Studying the Intercellular Communication of Neutrophils
Communication among neutrophils plays critical roles during various phases of inflammatory responses, with clinical relevance to both acute and chronic inflammatory diseases. Despite its significance, underlyi...
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Protocol
Isolation and Culture of Mouse Primary Microglia and Oligodendrocyte Precursor Cells
Microglia and oligodendrocyte precursor cells (OPCs) are critical glia subsets in the central nervous system (CNS) and are actively engaged in a body of diseases, such as stroke, Alzheimer’s disease, multiple ...
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Protocol
Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients
Mitochondria-associated membranes (MAMs) are regions where the endoplasmic reticulum (ER) interacts with mitochondria and regulate lipid trafficking, calcium signaling, ER stress, and inflammation activation. ...
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Protocol
TurboID-Based Proximity Labeling: A Method to Decipher Protein–Protein Interactions in Plants
Proteins form complex networks through interaction to drive biological processes. Thus, dissecting protein–protein interactions (PPIs) is essential for interpreting cellular processes. To overcome the drawback...
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Protocol
Immunophenoty**, Part II: Analysis of Nanoparticle Effects on the Composition and Activation Status of Human Peripheral Blood Mononuclear Cells
The use of nanoparticles as drug delivery carriers requires analysis of their safety, which among other tests, includes immunotoxicity. Nanoparticles are also increasingly used for applications intended to spe...
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Protocol
Gene Editing and Reprogramming of Human Fibroblast Cells to Human Induced Pluripotent Stem Cells
Predictive disease models play significant roles in advancing our knowledge of the pathology of human disease. In this field, animal models have been extensively employed and have provided crucial insights int...
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Protocol
T Cell Subsets and Immune Homeostasis
T cells are a heterogeneous group of cells that can be classified into different subtypes according to different classification methods. The body’s immune system has a highly complex and effective regulatory n...
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Protocol
Immunophenoty**, Part I: Instrument Calibration and Reagent Qualification for Immunophenoty** Analysis of Human Peripheral Blood Mononuclear Cell Cultures
Nanoparticles are increasingly used in biomedical applications to influence the way the immune system reacts to tumors and infectious disease-causing agents. Nanoparticles not-intended for immunomodulation can...
