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Protocol
Photoacoustic Tomography for Imaging Nanoparticles
Nanotechnology is the key to a new, noninvasive photoacoustic imaging technique that could detect early stages of disease tissues. The combination of photoacoustic imaging with nanotechnology holds promise for...
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Protocol
MRI and MRS of Human Brain Tumors
The purpose of this chapter is to provide an introduction to magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) of human brain tumors, including the primary applications and basic termi...
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Protocol
Identification of Tumor-Associated Antigens as Diagnostic and Predictive Biomarkers in Cancer
Many studies demonstrated that cancer sera contain antibodies which react with autologous cellular antigens generally known as tumor-associated antigens (TAAs). In our laboratories, the approach used in the id...
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Protocol
Methodologies in Assaying Prostate Cancer Stem Cells
The cancer stem cell (CSC) theory posits that only a small population of tumor cells within the tumor has the ability to reinitiate tumor development and is responsible for tumor homeostasis and progression. T...
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Protocol
Silencing Epidermal Growth Factor Receptor by RNA Interference in Glioma
Glioblastoma multiforme (GBM) can arise de novo or progress from a lower to higher grade and can possess a series of genetic alterations and dynamic progressions, which have been correlated with the molecular ...
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Protocol
Intercellular Adhesion Molecules (ICAM-1, VCAM-1, and LFA-1) in Adenocarcinoma of Lung
Cell adhesion molecules (CAMs) such as intercellular adhesion molecule (ICAM-1), vascular adhesion molecule (VCAM-1), and lymphocyte function-associated antigen (LFA-1) facilitate interaction among cells of va...
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Protocol
Antisense IGF-I for Hepatocellular Carcinoma
Antisense gene therapy involves the selective inhibition of expression or function of disease-causing genes by treatment with an antisense molecules (RNA or oligodeoxynucleotides) complementary to its target n...
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Protocol
p53 Plasmid Preparation and Techniques for Analysis of Gene Transfer and Expression
Hepatocellular carcinoma (HCC) is one of the most common causes of cancer death worldwide and is especially prevalent in certain areas of Africa and Asia. The most important etiological factor is infection wit...