5 Result(s)
within
Protocol
Erika L. F. Holzbaur
-
Protocol
Methods for Assessing Nuclear Rotation and Nuclear Positioning in Develo** Skeletal Muscle Cells
Skeletal muscle cells are large syncytia, containing hundreds of nuclei positioned regularly along the length of the fiber. During development, nuclei are actively distributed throughout the myotube by the mic...
-
Protocol
Imaging the Dynamics of Mitophagy in Live Cells
Investigating the precise spatiotemporal dynamics of mitophagy can provide insights into how mitochondrial quality control is regulated in different tissues and organisms. Here, we outline live imaging assays ...
-
Protocol
Live Imaging of Autophagosome Biogenesis and Maturation in Primary Neurons
Live-cell imaging of autophagy in primary neurons has revealed a robust and constitutive pathway for nonselective autophagy in the axon. Autophagosome biogenesis occurs in the distal axon; newly formed autopha...
-
Protocol
Employing Live-Cell Imaging to Study Motor-Mediated Transport
Microtubule-based transport is a highly regulated process, requiring kinesin and/or dynein motors, a multitude of motor-associated regulatory proteins including activating adaptors and scaffolding proteins, an...
-
Protocol
Single-Molecule Studies of Motor Adaptors Using Cell Lysates
Long-range transport of organelles and other cellular cargoes along microtubules is driven by kinesin and dynein motor proteins in complex with cargo-specific adaptors. While some adaptors interact exclusively...
