39 Result(s)
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Protocol
Structure-Guided and Phage-Assisted Evolution of Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated Resistance
Resistance to therapeutic antibodies caused by on-target point mutations is a major obstacle in anticancer therapy, creating an “unmet clinical need.” To tackle this problem, researchers are develo** new gen...
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Protocol
Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods
Phage-nanomaterial conjugates are functional bio-nanofibers with various applications. While phage display can select for phages with desired genetically encoded functions and properties, nanomaterials can end...
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Protocol
Enzymatic Preparation of DNA with an Expanded Genetic Alphabet Using Terminal Deoxynucleotidyl Transferase and Its Applications
Efficient preparation of DNA oligonucleotides containing unnatural nucleobases (UBs) that can pair with their cognates to form unnatural base pairs (UBPs) is an essential prerequisite for the application of UB...
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Protocol
Visualization of Engineered M13 Phages Bound to Bacterial Targets by Transmission Electron Microscopy
The filamentous phage M13 is one of the most well-studied and characterized phages, particularly since it was introduced as a scaffold for phage display, a technique to express and evolve fusion proteins on th...
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Protocol
Generation and Characterization of In Vitro Transcribed mRNA
Here we describe the in vitro preparation of mRNA from DNA templates, including setting up the transcription reaction, mRNA cap**, and mRNA labeling. We then describe methods used for mRNA characterization, ...
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Protocol
Construction of Xylose-Utilizing Cyanobacterial Chassis for Bioproduction Under Photomixotrophic Conditions
Xylose is a major component of lignocellulose and the second most abundant sugar present in nature after glucose; it, therefore, has been considered to be a promising renewable resource for the production of b...
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Protocol
Single-Cell Micropatterning by Non-fouling Hydrogels
Micropatterned substrate is a unique method for studying cell biology at the single-cell level. Using photolithography to create binary patterns of cell-adherent peptide surrounding by non-fouling cell-repelle...
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Protocol
Microfluidics-Enabled Isolation and Single-Cell Analysis of Circulating Tumor Cells
Microfluidic platforms enable the enrichment and analysis of circulating tumor cells (CTCs), a potential biomarker for cancer diagnosis, prognosis, and theragnosis. Combined with immunocytochemistry/immunofluo...
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Protocol
In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor
Bioreactors can offer an advanced platform to provide conditions that mimic the native microenvironment, which can also provide stretching environment for mechanobiology research. Tendon-derived stem cells (TD...
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Protocol
Methods to Measure the Inhibition of ABCG2 Transporter and Ferrochelatase Activity to Enhance Aminolevulinic Acid-Protoporphyrin IX Fluorescence-Guided Tumor Detection and Resection
Aminolevulinic acid (ALA) has been clinically used as an intraoperative fluorescence probe for protoporphyrin IX (PpIX) fluorescence-guided tumor resection and a PDT agent for cancer treatment. Although tumor ...
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Protocol
Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors
Extracellular vesicles (EVs) are particles with 100–1000 nm sizes which are secreted by cells for intercellular communication. Meanwhile, studies have found that EVs secreted by human stem cells carry similar ...
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Protocol
Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach
Overlap extension PCR is one of the routinely used methods to generate mutagenic genes for the functional and structural study of proteins. However, it is time-consuming to design the overlap** mutagenic pri...
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Protocol
Focused Ultrasound-Mediated Intranasal Brain Drug Delivery Technique (FUSIN)
The blood–brain barrier (BBB) is the major barrier for brain drug delivery and limits the treatment options for central nervous system diseases. To circumvent the BBB, we introduced the focused ultrasound-medi...
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Protocol
Site-Directed Mutagenesis Method Mediated by Cas9
This study presents an in vitro CRISPR/Cas9-mediated mutagenic (ICM) system that allows rapid construction of designed mutants or site-saturation mutagenesis libraries in a PCR-independent manner. The plasmid ...
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Protocol
Vitrification of Heart Valve Tissues
Application of the original vitrification protocol used for pieces of heart valves to intact heart valves has evolved over time. Ice-free cryopreservation by Protocol 1 using VS55 is limited to small samples (...
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Protocol
Vaccine Delivery with a Detoxified Bacterial Toxin
It is still a challenge to develop needle-free mucosal vaccines. Despite progress in the development of the influenza vaccine, it must be reformulated annually because of antigenic changes in circulating influ...
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Protocol
Bioprinting of Complex Vascularized Tissues
Functional vasculature is crucial for the maintenance of living tissues via the transport of oxygen, nutrients, and metabolic waste products. As a result, insufficient vascularization in thick engineered tissu...
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Protocol
Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria
Photosynthetic cyanobacteria are not only model organisms for studying photosynthesis and biological cycling of carbon in biosphere but also potential “green microbial factories” to produce renewable fuels and...
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Protocol
CdS Quantum Dots Modified Photoelectrochemical Biosensor for TATA-Binding Protein Probing
The photoelectrochemical (PEC) biosensor, in which light is utilized to excite the photoactive species and current is employed as the detection signal, is a newly appeared yet dynamically develo** technique ...
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Protocol
Detection of Tetanus Antibody Applying a Cu-Zn-In-S/ZnS Quantum Dot-Based Lateral Flow Immunoassay
Lateral flow test strip (LFTS) enables rapid, portable, and low-cost point-of-care testing (POCT) diagnosis. Quantum dots (QDs), which are fluorescent semiconductor nanocrystals with distinctive and unique pho...
