Abstract
Micropatterned substrate is a unique method for studying cell biology at the single-cell level. Using photolithography to create binary patterns of cell-adherent peptide surrounding by non-fouling cell-repellent poly(ethylene glycol) (PEG) hydrogel, this patterning method allows for controlling cell attachment with desired sizes and shapes up to 19 days. Here we provide the detailed procedure of fabrication for such patterns. This method will allow monitoring of prolonged reaction of single cells such as cell differentiation upon induction or time-resolved apoptosis stimulated by drug molecules for cancer treatment.
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Acknowledgments
T.-H. Chen and J. Ding are supported by NSFC/RGC Joint Research Scheme (grant no. 21961160721 from the National Natural Science Foundation of China and N_CityU119/19 from Research Grants Council of Hong Kong). The authors thank Miss **nlei Wang (Fudan University) and Dr. **ang Yao (Donghua University) for their ongoing support and advice.
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Liu, W., Chen, TH., Ding, J. (2023). Single-Cell Micropatterning by Non-fouling Hydrogels. In: Li, P.C., Wu, A.R. (eds) Single-Cell Assays. Methods in Molecular Biology, vol 2689. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3323-6_6
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DOI: https://doi.org/10.1007/978-1-0716-3323-6_6
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Online ISBN: 978-1-0716-3323-6
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