6 Result(s)
within
Protocol
Céline Legros
Life Sciences
-
Protocol
MT1 Receptor Signaling Pathways by Impedance Measurement
Melatonin exerts its classical effects of relay of the circadian rhythm through two G protein-coupled receptors, MT1 and MT2. The functions attributed to melatonin are so numerous that the action of this neuro...
-
Protocol
Functionality of Melatonin Receptors: Recruitment of β-Arrestin at MT1
The main process of downregulation of G protein-coupled receptors is desensitization by which the receptor is extruded from the membrane and directed to the endosomal compartment for recycling. Typically, th...
-
Protocol
Measuring Binding at the Putative Melatonin Receptor MT3
Melatonin, (N-acetyl-5-methoxytryptamine), is a neurohormone which possesses a wide range of biological effects. The effects mediated by melatonin are in part attributed to the antioxidant properties of the mo...
-
Protocol
Detection of Melatonin in Tissue Samples
Melatonin is present in higher quantity in brain during the night. The variation of its quantity is not only a matter of day/night cycle but also a matter of organ and tissues’ sublocalization. It is of the hi...
-
Protocol
Alternative Ligands at Melatonin Receptors
Melatonin (N-acetyl-5-methoxytryptamine) is a neurohormone that possesses a wide range of biological effects. Most of the main recognized effects of this hormone in mammals are due to its interaction with two ...
-
Protocol
Functionality of Melatonin Receptors: Internalization
The main step of classical desensitization of a receptor, by mean of its disappearance from the plasma membrane, is its internalization. This is a key factor in the regulation of agonist-mediated signaling pat...
