6 Result(s)
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Life Sciences
Chemistry/Food Science, general
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Protocol
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Protocol
Correction to: Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction
Chapter 10 of this book was published with the following errors, which have been corrected now.
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Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems
Cell-free protein synthesis (CFPS) enables the development of antibody conjugates, such as fluorophore conjugates and antibody-drug conjugates (ADCs), in a rapid and straightforward manner. In the first part, ...
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BeStSel: From Secondary Structure Analysis to Protein Fold Prediction by Circular Dichroism Spectroscopy
Far-UV circular dichroism (CD) spectroscopy is a classical method for the study of the secondary structure of polypeptides in solution. It has been the general view that the α-helix content can be estimated ac...
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Computational Enzyme Design at Zymvol
Directed evolution is the most recognized methodology for enzyme engineering. The main drawback resides in its random nature and in the limited sequence exploration; both require screening of thousands (if not...
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Genome-Wide Map** and Microscopy Visualization of Protein–DNA Interactions by pA-DamID
Several methods have been developed to map protein–DNA interactions genome-wide in the last decades. Protein A-DamID (pA-DamID) is a recent addition to this list with distinct advantages. pA-DamID relies on an...
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The Origin of Left-Handed Poly[d(G-C)]
The discovery of a reversible transition in the helical sense of a double-helical DNA was initiated by the first synthesis in 1967 of the alternating sequence poly[d(G-C)]. In 1968, exposure to high salt conce...
