9 Result(s)
-
Protocol
Genetic Encoding of a Fluorescent Noncanonical Amino Acid as a FRET Donor for the Analysis of Deubiquitinase Activities
The genetic code expansion technology enables the genetic encoding of fluorescent noncanonical amino acids (ncAAs) for site-specific fluorescent labeling of proteins. These co-translational and internal fluore...
-
Protocol
Protein Expression with Biosynthesized Noncanonical Amino Acids
Natural proteins are normally made by 20 canonical amino acids. Genetic code expansion (GCE) enables incorporation of diverse chemically synthesized noncanonical amino acids (ncAAs) by orthogonal aminoacyl-tRN...
-
Protocol
Use of Longitudinal Serum Analysis and Machine Learning to Develop a Classifier for Cancer Early Detection
Early detection of solid tumors through a simple screening process, such as the proteomic analysis of biofluids, has the potential to significantly alter the management and outcomes of cancers. The application...
-
Protocol
Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria
Photosynthetic cyanobacteria are not only model organisms for studying photosynthesis and biological cycling of carbon in biosphere but also potential “green microbial factories” to produce renewable fuels and...
-
Protocol
A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
Due to the advancements in modern medicine that have resulted in an increased number of immunocompromised individuals, the incidences and the associated mortality of invasive aspergillosis have continued to ri...
-
Protocol
A Wearable Sensing System for Assessment of Exposures to Environmental Volatile Organic Compounds
A portable chemical sensing system that integrates sample preconcentration, separation and detection as well as wireless communication functionalities into a compact, wearable format can provide continuous and...
-
Protocol
Comparative Transcriptome Analysis for Metabolic Engineering
Transcriptome profiling allows massively parallel analysis of the dynamic expression of all genes and captures the cell physiology and regulatory mechanism in a holistic manner. Compared to other “omic” techni...
-
Protocol
Identification of Mutations in Evolved Bacterial Genomes
Directed laboratory evolution is a common technique to obtain an evolved bacteria strain with a desired phenotype. This technique is especially useful as a supplement to rational engineering for complex phenot...
-
Protocol
Synthesis of Cbz-Protected Ketomethylene Dipeptide Isosteres
Ketomethylene peptide isosteres have been the focus of a variety of synthetic studies because of their potential as therapeutic agents in the treatment of medical conditions mediated by proteases (1–3). The dipep...
