408 Result(s)
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Protocol
Encoding Genetic Circuits with DNA Barcodes Paves the Way for High-Throughput Profiling of Dose-Response Curves of Metabolite Biosensors
Metabolite biosensors, through which the intracellular metabolite concentrations could be converted to changes in gene expression, are widely used in a variety of applications according to the different output...
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Protocol
Plant Expression of Trans-Encapsidated Chimeric Viral Vaccines with Animal RNA Replicons: An Update
In this protocol, we outline how to produce a chimeric viral vaccine in a biosafety level 1 (BSL1) environment. An animal viral vector RNA encapsidated with tobacco mosaic virus (TMV) coat protein can be fully...
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Protocol
Two-Photon FRET/FLIM Imaging of Cerebral Neurons
Two-photon FRET (Förster resonance energy transfer) and FLIM (fluorescence lifetime imaging microscopy) enable the detection of FRET changes of fluorescence reporters in deep brain tissues, which provide a val...
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Protocol
Detection of dsRNA with Fluorescence In Situ Hybridization (FISH)
Fluorescence in situ hybridization (FISH) is a powerful technique used for detecting and localizing specific nucleic acid sequences in cells or tissues. Double-stranded RNA (dsRNA) is a type of RNA with comple...
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Protocol
Extraction of Proteins from Soil
Soil metaproteomics could explore the proteins involved in life activities and their abundance in the soils to overcome the difficulty in pure cultures of soil microorganisms and the limitations of proteomics ...
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Protocol
Nano-optogenetic CAR-T Cell Immunotherapy
Chimeric antigen receptor (CAR)-T cell immunotherapy emerges as an effective cancer treatment. However, significant safety concerns remain, such as cytokine release syndrome (CRS) and “on-target, off-tumor” cy...
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Protocol
Isolation of Mitochondria from Murine Skeletal Muscle
Skeletal muscle is one of the largest tissues in human body. Besides enabling voluntary movements and maintaining body’s metabolic homeostasis, skeletal muscle is also a target of many pathological conditions....
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Protocol
Fungal Secretomics Through iTRAQ-Based Analysis
Our understanding of how fungi respond and adapt to external environments can be increased by the comprehensive data sets of fungal-secreted proteins. Fungi produce a variety of secreted proteins, and environm...
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Protocol
A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in Fusobacterium nucleatum
Controlled septal peptidoglycan hydrolysis is vital for bacterial cell division, preserving cellular integrity and facilitating proper daughter cell separation. In Escherichia coli, the FtsEX ABC system governs c...
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Protocol
DeepHLApan: A Deep Learning Approach for the Prediction of Peptide-HLA Binding and Immunogenicity
Neoantigens are crucial in distinguishing cancer cells from normal ones and play a significant role in cancer immunotherapy. The field of bioinformatics prediction for tumor neoantigens has rapidly developed, ...
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Protocol
Characterization of T-Cell Epitopes in Food Allergens by Bioinformatic Tools
The identification of T-cell epitopes is a critical step in the understanding of the immunologic mechanisms such as food allergy. Epitope screening in silico by bioinformatic tools can be used to identify T-ce...
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Protocol
Rapid Screening of CAR T Cell Functional Improvement Strategies by Highly Multiplexed Single-Cell Secretomics
The functional fitness of CAR T cells plays a crucial role in determining their clinical efficacy. Several strategies are being explored to increase cellular fitness, but screening these approaches in vivo is ...
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Protocol
Exploring the Archaeal Virosphere by Metagenomics
During the past decade, environmental research has demonstrated that archaea are abundant and widespread in nature and play important ecological roles at a global scale. Currently, however, the majority of arc...
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Protocol
Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System
Antibodies specifically recognizing integral membrane proteins are essential tools for functional analysis, diagnosis, and therapeutics targeting membrane proteins. However, develo** antibodies against membr...
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Protocol
Identification of Unambiguous Borrelia Peptides in Human Urine Using Affinity Capture and Mass Spectrometry
The combination of advanced mass spectrometry and enrichment-based sample preparation methods has enhanced analytical capabilities in clinical proteomics. In this chapter, we describe a method of proteome anal...
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Protocol
Data Analysis Pipeline for scRNA-seq Experiments to Study Early Oogenesis
Germ cells as the means for the transmission of genetic information between generations have been a hot topic of research for decades. The analysis of the transcriptomes, that is of the RNA transcripts produce...
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Protocol
Label-Free Quantitative Proteomics in Plant
Label-free quantitation (LFQ) proteomics, mainly based on the extraction of the peptide (precursor) intensity at the MS1 (mass spectrum 1) level, enables to quantify the relative amount of the proteins among samp...
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Protocol
High-Throughput Base Editing-Mediated Artificial Evolution Streamlines Trait Gene Identification in Rice
Base Editing-Mediated Gene Evolution (BEMGE) method develops novel rice germplasms with mutations in any endogenous gene through employing both Cas9n-based cytosine and adenine base editors combined with an sg...
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Protocol
Confocal Laser Scanning Imaging of Cell Junctions in Human Colon Cancer Cells
The intestinal epithelium is formed by a single layer of cells. These cells originate from self-renewal stem cells that give rise to various lineages of cells: Paneth, transit-amplifying, and fully differentia...
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Protocol
High-Throughput and In-Depth Proteomic Profiling of 5 μL Plasma and Serum Using TMTpro 16-Plex
High-throughput and in-depth proteomic analysis of plasma and serum samples remains challenging due to the presence of multiple high-abundance proteins. Here, we provide a detailed protocol for proteomic analy...
