8 Result(s)
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Protocol
Nanofluidic Channel Fabrication and Manipulation of DNA Molecules
Confining DNA molecules in a nanofluidic channel, particularly in channels with cross sections comparable to the persistence length of the DNA molecule (about 50 nm), allows the discovery of new biophysical ph...
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Protocol
The Monitoring and Affinity Purification of Proteins Using Dual Tags with Tetracysteine Motifs
Identification and characterization of protein-protein interaction networks is essential for the elucidation of biochemical mechanisms and cellular function. Affinity purification in combination with liquid ch...
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Protocol
Temperature and pH-Responsive “Smart” Carbon Nanotube Dispersions
Carbon nanotubes (CNTs) are a family of all-carbon quasi one-dimensional nanomaterials that are highly hydrophobic and typically aggregated in bundles. Recent accomplishments in dispersing CNTs in aqueous solu...
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Protocol
Design of an Intron-Retained Bioluminescence Reporter and its Application in Imaging of Pre-mRNA Splicing in Living Subjects
Aberrant splicing of precursor messenger RNA (pre-mRNA) can generate abnormal transcripts, and most of the human diseases have been shown to associate with abnormal splicing of pre-mRNA. Conventional methods r...
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Protocol
Mix-and-Inject Serial Femtosecond Crystallography to Capture RNA Riboswitch Intermediates
Time-resolved structure determination of macromolecular conformations and ligand-bound intermediates is extremely challenging, particularly for RNA. With rapid technological advances in both microfluidic liqui...
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Protocol
Combining Biophysical Methods for Structure–Function Analyses of RNA in Solution
RNA-level regulation by riboswitches relies on the specific binding of small metabolites to the aptamer domain to trigger substantial conformational changes that affect transcription or translation. Although s...
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Protocol
Combining Experimental Restraints and RNA 3D Structure Prediction in RNA Nanotechnology
Precise RNA tertiary structure prediction can aid in the design of RNA nanoparticles. However, most existing RNA tertiary structure prediction methods are limited to small RNAs with relatively simple secondary...
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Protocol
Reconstructing Signaling Networks Using Biosensor Barcoding
Understanding how signaling networks are regulated offers valuable insights into how cells and organisms react to internal and external stimuli and is crucial for develo** novel strategies to treat diseases....
