11 Result(s)
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Protocol
Automated Analysis of Intracellular Dynamic Processes
The study of intracellular dynamic processes is of fundamental importance for understanding a wide variety of diseases and develo** effective drugs and therapies. Advanced fluorescence microscopy imaging sys...
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Protocol
Isolation of Exosomes from HTLV-Infected Cells
Exosomes are small vesicles, approximately 30–100 nm in diameter, that transport various cargos, such as proteins and nucleic acids, between cells. It has been previously shown that exosomes can also transport...
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Protocol
Role of Immune Aging in Susceptibility to West Nile Virus
West Nile virus (WNV) can cause severe neuroinvasive disease in humans and currently no vaccine or specific treatments are available. As aging is the most prominent risk factor for WNV, age-related immune dysr...
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Protocol
Systems Biology Approach to Imaging of Neural Stem Cells
Over the past decade, the advances in human brain magnetic resonance imaging (MRI) have significantly improved our ability to gain insightful information about the structure and function of the brain. One of t...
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Protocol
Computing Genetic Imprinting Expressed by Haplotypes
Different expression of maternally and paternally inherited alleles at certain genes is called genetic imprinting. Despite its great importance in trait formation, development, and evolution, it remains unclea...
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Protocol
Modeling Cerebral Ischemia in Neuroproteomics
Protein changes induced by traumatic or ischemic brain injury can serve as diagnostic markers as well as therapeutic targets for neuroprotection. The focus of this chapter is to provide a representative overvi...
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Protocol
Autologous Mesenchymal Stem Cells for Post-Ischemic Myocardial Repair
Cell transplantation is a novel therapy for patients with postinfarction ven tricular dysfunction and congestive heart failure. The potential of mesenchy mal stem cells (MSCs) to be used in the injured myocard...
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Protocol
Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failure
Cardiomyocytes are final differentiated cells that lose the ability to regen erate. Autologous cellular transplantation for cardiac repair has recently emerged as a promising new approach for end-stage heart f...
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Protocol
Animal Models of Cardiac Fibrosis
A collagen network, composed largely of type I and III fibrillar collagens, is found in the heart’s interstitial space. This network has multiple functions, including the preservation of tissue architecture an...
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Protocol
Localization of Duck Hepatitis B Virus Polymerase Within Cells
Hepadnaviruses are small, DNA-containing viruses that replicate by reverse transcription (1). They have a lipid envelope surrounding an icosahedral protein core particle, whose shell is composed of a single viral...
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Protocol
Anti-Nitrotyrosine Antibodies for Immunohistochemistry
Nitrotyrosine is an important marker for the formation of peroxynitrite and possibly other reactive nitrogen species derived from nitric oxide in vivo (1). Pathological conditions can substantially increase the p...
