Abstract
Hepadnaviruses are small, DNA-containing viruses that replicate by reverse transcription (1). They have a lipid envelope surrounding an icosahedral protein core particle, whose shell is composed of a single viral protein, the core protein. Within the core particle, the viral reverse transcriptase (polymerase) is covalently linked to the double-stranded viral genome.
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References
Ganem, D. and Schneider, R. J. (2001) Hepadnaviridae: the viruses and their replication. In: Knipe, D. M. Howley, P. M. Griffen, D. E. et al. (eds.), Fields Virology, 4th ed., vol 2, Lippencott Williams & Wilkins, Philadelphia, PA, 2923–2969.
Bartenschlager, R., Junker-Niepmann, M., and Schaller, H. (1990) The P gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidation. J. Virol. 64, 5324–5332.
Junker-Niepmann, M., Bartenschlager, R., and Schaller, H. (1990) A short cis-acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA. EMBO J. 9, 3389–3396.
Hirsch, R. C., Lavine, J. E. Chang, L. J. Varmus, H. E. and Ganem, D. (1990) Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as well as for reverse transcription. Nature 344, 552–555.
Tavis, J. E. and Ganem, D. (1996) Evidence for the activation of the hepatitis B virus polymerase by binding of its RNA template. J. Virol. 70, 5741–5750.
Tavis, J. E., Massey, B., and Gong, Y. (1998) The duck hepatitis B virus polymerase is activated by its RNA packaging signal, epsilon. J. Virol. 72, 5789–5796.
Wang, G.-H. and Seeger, C. (1993) Novel mechanism for reverse transcription in hepatitis B viruses. J. Virol. 67, 6507–6512.
Tavis, J. E., Perri, S., and Ganem, D. (1994) Hepadnavirus reverse transcription initiates within the stem-loop of the RNA packaging signal and employs a novel strand transfer. J. Virol. 68, 3536–3543.
Tavis, J. E. and Ganem, D. (1995) RNA sequences controlling the initiation and transfer of duck hepatitis B virus minus-strand DNA. J. Virol. 69, 4283–4291.
Nassal, M. and Rieger, A. (1996) A bulged region of the hepatitis B virus RNA encapsidation signal contains the replication origin for discontinuous first-strand DNA synthesis. J. Virol. 70, 2764–2773.
Chang, L.-J., Hirsch, R. C., Ganem, D., and Varmus, H. E. (1990) Effects of insertional and point mutations on the functions of the duck hepatitis B virus polymerase. J. Virol. 64, 5553–5558.
Radziwill, G., Tucker, W., and Schaller, H. (1990) Mutational analysis of the hepatitis B virus P gene product: domain structure and RNase H activity. J. Virol. 64, 613–620.
Zoulim, F. and Seeger, C. (1994) Reverse transcription in hepatitis B viruses is primed by a tyrosine residue of the polymerase. J. Virol. 68, 6–13.
Weber, M., Bronsema, V., Bartos, H., Bosserhoff, A., Bartenschlager, R., and Schaller, H. (1994) Hepadnavirus P protein utilizes a tyrosine residue in the TP domain to prime reverse transcription. J. Virol. 68, 2994–2999.
McClure, M. A. (1993) Evolutionary history of reverse transcriptase. In: Skalka, A. M. and Goff, S. P. (eds.), Reverse Transcriptase, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 425–444.
Poch, O., Sauvaget, I., Delarue, M., and Tordo, N. (1989) Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO J. 8, 3867–3874.
Li, M. D., Bronson, D. L., Lemke, T. D., and Faras, A. J. (1995) Phylogenetic analyses of 55 retroelements on the basis of the nucleotide and product amino acid sequences of the pol gene. Mol. Biol. Evol. 12, 657–670.
Bavand, M., Feitelson, M., and Laub, O. (1989) The hepatitis B virus-associated reverse transcriptase is encoded by the viral pol gene. J. Virol. 63, 1019–1021.
Mack, D. H., Bloch, W., Nath, N., and Sninsky, J. J. (1988) Hepatitis B virus particles contain a polypeptide encoded by the largest open reading frame: a putative reverse transcriptas. J. Virol. 62, 4786–4790.
Bartenschlager, R. and Schaller, H. (1988) The amino-terminal domain of the hepadnaviral P-gene encodes the terminal protein (genome-linked protein) believed to prime reverse transcription. EMBO J. 7, 4185–4192.
Huang, H.-L., Jeng, K.-S., Hu, C.-P., Tsai, C.-H., Lo, S. J., and Chang, C. (2000) Identification and characterization of a structural protein of hepatitis B virus: a polymerase and surface fusion protein encoded by a spliced RNA. Virology 275, 398–410.
Bartenschlager, R., Kuhn, C., and Schaller, H. (1992) Expression of the P-protein of the human hepatitis B virus in a vaccinia virus system and detection of the nucleocapsid-associated P-gene product by radiolabelling at newly introduced phosphorylation sites. Nucl. Acids Res. 20, 195–202.
Oberhaus, S. M. and Newbold, J. E. (1996) Preparations of duck hepatitis B virions contain multiple DNA polymerase activities. Virology 226, 132–134.
Oberhaus, S. M. and Newbold, J. E. (1993) Detection of DNA polymerase activities associated with purified duck hepatitis B virus core particles by using an activity gel assay. J. Virol. 67, 6558–6566.
Bavand, M. R. and Laub, O. (1988) Two proteins with reverse transcriptase activities associated with hepatitis B virus-like particles. J. Virol. 62, 626–628.
zu Putlitz, J., Lanford, R. E., Carlson, R. I., Notvall, L., De la Monte, S. M. and Wands, J. R. (1999) Properties of monoclonal antibodies directed against hepatitis B virus polymerase protein. J. Virol. 73, 4188–4196.
Yao, E., Gong, Y., Chen, N., and Tavis, J. E. (2000) The majority of duck hepatitis B virus reverse transcriptase in cells is nonencapsidated and is bound to a cytoplasmic structure. J. Virol. 74, 8648–8657.
Radziwill, G., Zentgraf, H., Schaller, H., and Bosch, V. (1988) The duck hepatitis B virus DNA polymerase is tightly associated with the viral core structure and unable to switch to an exogenous template. Virology 163, 123–132.
Sprengel, R., Kuhn, C., Will, H., and Schaller, H. (1985) Comparative sequence analysis of duck and human hepatitis B virus genomes. J. Med. Virol. 15, 323–333.
Condreay, L. D., Aldrich, C. E., Coates, L., Mason, W. S., and Wu, T.-T. (1990) Efficient duck hepatitis B virus production by an avian liver tumor cell line. J. Virol. 64, 3249–3258.
Pollack, J. R. and Ganem, D. (1994) Site-specific RNA binding by a hepatitis B virus reverse transcriptase initiates two distinct reactions: RNA packaging and DNA synthesis. J. Virol. 68, 5579–5587.
Kozak, M. (1991) Structural features in eukaryotic mRNAs that modulate the initiation of translation. J. Biol. Chem. 266, 19,867–19,870.
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Yao, E., Tavis, J.E. (2004). Localization of Duck Hepatitis B Virus Polymerase Within Cells. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicine, vol 95. Humana Press. https://doi.org/10.1385/1-59259-669-X:281
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DOI: https://doi.org/10.1385/1-59259-669-X:281
Publisher Name: Humana Press
Print ISBN: 978-1-58829-105-9
Online ISBN: 978-1-59259-669-0
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