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315 Result(s)
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Chapter and Conference Paper
An Influence of Plant Growth Substances on Growth and Nitrogenase Activity from Azospirillum Brasiliense
Plant growth substances, such as auxins, cytocinin, gibberillin and abciscin were tested for their effect on growth and nitrogenase activity from batch culture grown Azospirillum brasilense. The present results i...
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Chapter and Conference Paper
Quantitative Measurement of Azospirillum Plant Cell Attachment
A quantitative plant assay system is described for measuring the attachment of Azospirillum spp. to plant cells. The attachment of different Azospirillum strains was assayed by mixing 14C-leucine labeled bacteria...
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Chapter and Conference Paper
Tn5-Mutagenesis in Azospirillum Brasilense
A system is described for transposon mutagenesis of Azospirillum brasilense using the mobilizable plasmid pSUP2021. Mutants were selected on rich medium containing kanamycin and streptomycin. All the auxotrophs i...
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Chapter and Conference Paper
Nitrogenase Activity of Sterile Cereal Seedlings Inoculated with Azospirillum Brasilense: Differences Between Cultivars and Possible Significance
Large differences in supporting nitrogenase activity existed between 14 German cereal cultivars. Fractions of active plants ranged between 3 and 67% and maximal activities between 5 and 35 nmol C2H4.h−1. No signi...
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Chapter and Conference Paper
Aromatic Amino Acid Aminotransferases of Azospirillum Lipoferum and Their Possible Involvement in IAA Biosynthesis
Four aromatic amino acid aminotransferases were identified in A. lipoferum ATCC 29708. Each enzyme can in vitro catalyze the transamination of tryptophan, which is the initial reaction in the biosynthesis of IAA ...
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Chapter and Conference Paper
Aspects of the Ecology of Azospirillum sp. in Belgian Soils
Azospirillum sp. was present in most of the Belgian fields. However it was never present in concentrations exceeding 103 cfu per gram of soil. Under field conditions none of the known species was fou...
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Chapter and Conference Paper
Azospirilla from Rice Fields Near Hanoi
Azospirilla are frequent in the rhizosphere of rice plants in Tu-liem district. Isolates obtained were either typical Azospirilla or had at least some characters of Azospirilla. Members of both groups had bene...
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Chapter and Conference Paper
A Transformable Mutant of Azosprillum Brasilense ATCC 29710
Azospirilla are normally not transformable. During our investigations a mutant of A. brasilense ATCC 29710 was isolated, which was transformable with plasmid-DNA (e.g. pSUP106 and pRK290). The mutant also was a b...
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Chapter and Conference Paper
Azospirillum spp Ecology of Some Soils of the Somali Republic
During a survey of the distribution of nitrogen-fixing bacteria in soil samples (12 alluvial and 1 sandy) collected in two localities (Jenale and Afgoj) in the Somali Republic, microorganisms resembling Azospiril...
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Chapter and Conference Paper
Plasmid Transfer in Soil and Rhizosphere
Transfer of plasmids pFT30 and RP4 between newly-introduced bacilli and pseudomonads, respectively, is described. In sterile soil, transfer of pFT30 was only detected when exogenous nutrients were added; bento...
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Chapter and Conference Paper
Rhizobium Leguminosarum as a Model for Investigating Gene Transfer in Soil
A conjugative symbiotic plasmid of Rhizobium leguminosarum bv. viceae was marked with transposon Tn5 and the strain was used to inoculate peas in the field. The Tn5 marker could be readily identified in rhizob...
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Chapter and Conference Paper
Impact of Mineral Surfaces on Gene Transfer by Transformation in Natural Bacterial Environments
We have developed a microenvironmental system consisting of glass columns filled with quartz-rich chemically pure sea sand. The columns were percolated with medium. Using this system, we have determined parame...
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Chapter and Conference Paper
Introduction of Rhizobium into Soils
Preliminary results of the common experiment designed to investigate gene transfer in the environment between introduced Rhizobium leguminosarum bv viceae RSM2004 and native strains of bacteria are reported. T...
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Chapter and Conference Paper
Biological Containment of Bacteria and Plasmids to be Released in the Environment
The design of a biological containment system to be employed in a broad spectrum of bacteria useful in connection with release to the external evironment is reported. The key element is a gene, hok, encoding a...
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Chapter and Conference Paper
Root Hair Deformation Induced on Maize and Medicago by an Azospirillum Transconjugant Containing a Rhizobium Meliloti Nodulation Region
Rhizobium bacteria form nitrogen-fixing root nodules on legume plants. This process requires the induction and expression of genes in both partners. After cloning of the nod hsn region from the pSym ...
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Chapter and Conference Paper
Preliminary Trials of Field Release of Azospirillum Brasilense as Inoculant in Northern Italy
Two fields experiments were conducted with Triticum durum during 1985/86 and 1986/87 in northern Italy, to study inoculation effects of homologous and heterologous strains of Azospirillum brasilense.
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Chapter and Conference Paper
Studies on the Fate and Genetic Stability of Recombinant Microorganisms in Model Ecosystems
Risk assessment studies concering deliberate release of recombinant microorganisms were performed in micro-ecosystems. For this purpose chemostate and microcosm model systems were designed comprising plain soi...
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Chapter and Conference Paper
The Approach of the U.S. Environmental Protection Agency in Regulating Certain Biotechnology Products
The U.S. Environmental Protection Agency functions under a number of statutes to carry out its mission of protecting human health and the environment. While these statutes were not written specifically for bio...
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Chapter and Conference Paper
Are Azospirillum Bacteriocins Produced and Active in Soil?
Five different soils and 3 soil components were treated with bacte riocin produced by A. lipoferum 215. The activity disappeared soon after application in four soils and in humic acid preparation, but persisted i...
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Chapter and Conference Paper
Safety of Baculoviruses used as Biological Insecticides
About 1100 viruses are known to infect insects, more than 60% of them being baculoviruses (1). The members of the virus family Baculoviridae are characterized by double stranded circular DNA, included in rod-s...