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    Chapter and Conference Paper

    An Influence of Plant Growth Substances on Growth and Nitrogenase Activity from Azospirillum Brasiliense

    Plant growth substances, such as auxins, cytocinin, gibberillin and abciscin were tested for their effect on growth and nitrogenase activity from batch culture grown Azospirillum brasilense. The present results i...

    C. Christiansen-Weniger in Azospirillum IV (1988)

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    Chapter and Conference Paper

    Quantitative Measurement of Azospirillum Plant Cell Attachment

    A quantitative plant assay system is described for measuring the attachment of Azospirillum spp. to plant cells. The attachment of different Azospirillum strains was assayed by mixing 14C-leucine labeled bacteria...

    M. Eyers, F. Waelkens, J. Vanderleyden, A. P. Van Gool in Azospirillum IV (1988)

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    Chapter and Conference Paper

    Tn5-Mutagenesis in Azospirillum Brasilense

    A system is described for transposon mutagenesis of Azospirillum brasilense using the mobilizable plasmid pSUP2021. Mutants were selected on rich medium containing kanamycin and streptomycin. All the auxotrophs i...

    M. Vanstockem, A. Milcamps, K. Michiels, J. Vanderleyden, A. P. Van Gool in Azospirillum IV (1988)

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    Chapter and Conference Paper

    Nitrogenase Activity of Sterile Cereal Seedlings Inoculated with Azospirillum Brasilense: Differences Between Cultivars and Possible Significance

    Large differences in supporting nitrogenase activity existed between 14 German cereal cultivars. Fractions of active plants ranged between 3 and 67% and maximal activities between 5 and 35 nmol C2H4.h−1. No signi...

    G. Jagnow in Azospirillum IV (1988)

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    Chapter and Conference Paper

    Aromatic Amino Acid Aminotransferases of Azospirillum Lipoferum and Their Possible Involvement in IAA Biosynthesis

    Four aromatic amino acid aminotransferases were identified in A. lipoferum ATCC 29708. Each enzyme can in vitro catalyze the transamination of tryptophan, which is the initial reaction in the biosynthesis of IAA ...

    E. Ruckdäschel, B. Lewis Kittell, D. R. Helinski, U. Klingmüller in Azospirillum IV (1988)

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    Chapter and Conference Paper

    Aspects of the Ecology of Azospirillum sp. in Belgian Soils

    Azospirillum sp. was present in most of the Belgian fields. However it was never present in concentrations exceeding 103 cfu per gram of soil. Under field conditions none of the known species was fou...

    S. Horemans, K. De Coninck, K. Vlassak in Azospirillum IV (1988)

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    Chapter and Conference Paper

    Azospirilla from Rice Fields Near Hanoi

    Azospirilla are frequent in the rhizosphere of rice plants in Tu-liem district. Isolates obtained were either typical Azospirilla or had at least some characters of Azospirilla. Members of both groups had bene...

    Nguyen Ngoc Dung, Nguyen phuong Chi, Ly kim Bang in Azospirillum IV (1988)

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    Chapter and Conference Paper

    A Transformable Mutant of Azosprillum Brasilense ATCC 29710

    Azospirilla are normally not transformable. During our investigations a mutant of A. brasilense ATCC 29710 was isolated, which was transformable with plasmid-DNA (e.g. pSUP106 and pRK290). The mutant also was a b...

    H. El-Khawas, W. Klingmüller in Azospirillum IV (1988)

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    Chapter and Conference Paper

    Azospirillum spp Ecology of Some Soils of the Somali Republic

    During a survey of the distribution of nitrogen-fixing bacteria in soil samples (12 alluvial and 1 sandy) collected in two localities (Jenale and Afgoj) in the Somali Republic, microorganisms resembling Azospiril...

    F. Favilli, F. Trinci, W. Balloni in Azospirillum IV (1988)

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    Chapter and Conference Paper

    Plasmid Transfer in Soil and Rhizosphere

    Transfer of plasmids pFT30 and RP4 between newly-introduced bacilli and pseudomonads, respectively, is described. In sterile soil, transfer of pFT30 was only detected when exogenous nutrients were added; bento...

    Jan Dirk van Elsas, Jack T. Trevors in Risk Assessment for Deliberate Releases (1988)

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    Chapter and Conference Paper

    Rhizobium Leguminosarum as a Model for Investigating Gene Transfer in Soil

    A conjugative symbiotic plasmid of Rhizobium leguminosarum bv. viceae was marked with transposon Tn5 and the strain was used to inoculate peas in the field. The Tn5 marker could be readily identified in rhizob...

    Penny R. Hirsch, John R. Spokes in Risk Assessment for Deliberate Releases (1988)

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    Chapter and Conference Paper

    Impact of Mineral Surfaces on Gene Transfer by Transformation in Natural Bacterial Environments

    We have developed a microenvironmental system consisting of glass columns filled with quartz-rich chemically pure sea sand. The columns were percolated with medium. Using this system, we have determined parame...

    Michael G. Lorenz, Wilfried Wackernagel in Risk Assessment for Deliberate Releases (1988)

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    Chapter and Conference Paper

    Introduction of Rhizobium into Soils

    Preliminary results of the common experiment designed to investigate gene transfer in the environment between introduced Rhizobium leguminosarum bv viceae RSM2004 and native strains of bacteria are reported. T...

    Noëlle Amarger in Risk Assessment for Deliberate Releases (1988)

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    Chapter and Conference Paper

    Biological Containment of Bacteria and Plasmids to be Released in the Environment

    The design of a biological containment system to be employed in a broad spectrum of bacteria useful in connection with release to the external evironment is reported. The key element is a gene, hok, encoding a...

    S. Molin, P. Klemm, L. K. Poulsen, H. Biehl in Risk Assessment for Deliberate Releases (1988)

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    Chapter and Conference Paper

    Root Hair Deformation Induced on Maize and Medicago by an Azospirillum Transconjugant Containing a Rhizobium Meliloti Nodulation Region

    Rhizobium bacteria form nitrogen-fixing root nodules on legume plants. This process requires the induction and expression of genes in both partners. After cloning of the nod hsn region from the pSym ...

    L. Piana, M. Delledonne, M. N. Antonelli, C. Fogher in Azospirillum IV (1988)

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    Chapter and Conference Paper

    Preliminary Trials of Field Release of Azospirillum Brasilense as Inoculant in Northern Italy

    Two fields experiments were conducted with Triticum durum during 1985/86 and 1986/87 in northern Italy, to study inoculation effects of homologous and heterologous strains of Azospirillum brasilense.

    M. P. Nuti, P. Rubboli in Risk Assessment for Deliberate Releases (1988)

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    Chapter and Conference Paper

    Studies on the Fate and Genetic Stability of Recombinant Microorganisms in Model Ecosystems

    Risk assessment studies concering deliberate release of recombinant microorganisms were performed in micro-ecosystems. For this purpose chemostate and microcosm model systems were designed comprising plain soi...

    F. R. J. Schmidt, R. Henschke, E. Nücken in Risk Assessment for Deliberate Releases (1988)

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    Chapter and Conference Paper

    The Approach of the U.S. Environmental Protection Agency in Regulating Certain Biotechnology Products

    The U.S. Environmental Protection Agency functions under a number of statutes to carry out its mission of protecting human health and the environment. While these statutes were not written specifically for bio...

    Elizabeth A. Milewski Ph. D. in Risk Assessment for Deliberate Releases (1988)

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    Chapter and Conference Paper

    Are Azospirillum Bacteriocins Produced and Active in Soil?

    Five different soils and 3 soil components were treated with bacte riocin produced by A. lipoferum 215. The activity disappeared soon after application in four soils and in humic acid preparation, but persisted i...

    R. G. B. Oliveira, A. Drozdowicz in Azospirillum IV (1988)

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    Chapter and Conference Paper

    Safety of Baculoviruses used as Biological Insecticides

    About 1100 viruses are known to infect insects, more than 60% of them being baculoviruses (1). The members of the virus family Baculoviridae are characterized by double stranded circular DNA, included in rod-s...

    Jürg Huber in Risk Assessment for Deliberate Releases (1988)

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