22 Result(s)
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Chapter
Harinder P. S. Makkar
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Chapter
Phytochemicals in Jatropha Seeds and Potential Agro-Pharmaceutical Applications of Jatropha curcas Phorbol Esters
Jatropha curcas (Euphorbiaceae) is a promising energy plant since its seeds are rich in oil (23–37 %) and the biodiesel produced on esterification of the oil meets the European and American biodies...
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In Vitro Screening of Feed Resources for Efficiency of Microbial Protein Synthesis
Recent advances in ration balancing include manipulation of feed to increase the quantity and quality of protein and energy delivered to the small intestine. Proportionally high conversion of feed degraded in ...
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Screening Plants and Plant Products for Methane Inhibitors
Plant secondary compounds may have important antimicrobial activity and these compounds have been suggested as alternative additives for use in ruminant feeding. The plant secondary compounds may modify rumina...
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Current situation and prospects of Jatropha curcas as a multipurpose tree in China
This paper reviews the current status of studies on Jatropha curcas in China. Jatropha curcas has been grown in China for more than 300 years. It is mainly distributed in the southwest from the Yunnan-Guizhou Pla...
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Chapter and Conference Paper
Effect of Secondary Compounds in Forages on Rumen Micro-organisms Quantified by 16S And 18S rRNA
A gas syringe method was used to evaluate the effect of secondary compounds from plant materials on in vitro fermentation products and microbial biomass. The experiment used Pennisetum purpureum, Morinda citrifol...
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Determination of Condensed Tannins (Proanthocyanidins)
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Gallotannin Determination Using HPLC
Pipette 1 ml of supernatant A (see Section 5) into the above mentioned culture tubes, remove acetone in a vacuum oven adjusted at 40°C (pressure 300 mbar). It takes about 3 h to remove acetone. Acetone can also b...
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Treatment of Plant Material, Extraction of Tannins, and an Overview of Tannin Assays Presented in the Manual
Tannins are naturally occurring polyphenolic substances with various molecular weights and a variable complexity. These are chemically not well-defined substances but rather a group of substances with the abil...
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Tannin Bioassay
Dried leaves should be passed through a 1 mm sieve.
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Simplified Radiolabelled BSA Precipitation Method
The above radiolabelled method hass been simplified to eliminate the more difficult procedures of relatively high speed (13,000 g) centrifugation and manipulations of small volumes of radiochemical solutions by b...
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Determination of Protein-Precipitable Phenolics
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References
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Measurement of Total Phenolics and Tannins Using Folin-Ciocalteu Method
The method for total phenol is useful in order to know the efficiency of extraction of phenolics in solvents. This method can be coupled with the use of insoluble matrix, polyvinyl polypyrrolidone (PVPP; binds...
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Radial Diffusion Assay
Type I, gel point 36°C (Sigma A-6013), bovine serum albumin (fraction V) (Sigma A-3350), glacial acetic acid, ascorbic acid and sodium hydroxide.
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Gallotannin Determination by Rhodanine Assay
The plant material was extracted as described in Section 2.2.4. The supernatant is designated as supernatant A.
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Radiolabelled BSA Precipitation Method
This is the most sensitive method for determining amounts of protein precipitated by a tannin. It is also useful for running competitive binding assay to characterize tannin-protein interactions. The method de...
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Determination of Hydrolysable Tannins (Gallotannins and Ellagitannins) after Reaction with Potassium Iodate
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Characterization of Phenolic Compounds by Thin Layer Chromatography (TLC)
About 500 g of the shade dried plant material should be ground first to pass a 2 mm screen. All the ground material including those parts remaining inside the mill should be taken, mixed well and approximately...
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Protein-Binding Capacity by Filter Paper Assay
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Summary of Results from the First Phase of the Co-Ordinated Research Project
This CRP was initiated in 1999. The Second Research Coordination Meeting was held from 19 to 23 November 2001 at Centro de Energia Nuclear na Agricultura (CENA), Universidade de Sao Paulo, Piracicaba, Brazil, ...
