133 Result(s)
within
Chapter
**-** Wang
Biochemistry, general
Cell Biology
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Chapter
Chemical Probes for the Geometry of Membrane Proteins
The position of the protein components of biological membranes both perpendicular to and in the plane of the permeability barrier has been the subject of much recent interest. The “sidedness” of the identifiab...
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Chapter and Conference Paper
Physiological and Biochemical Aspects of Mammalian Hibernation
Hibernation in mammals in response to cold and food shortage in winter is characterized by a profound reduction in metabolism (to 1% or less of normal) and body temperature (to near 0 °C) which may last from a...
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Chapter
Dynamic Interactions of the Second Messenger Systems
The response of living cells to changes in cell environment depends on the production and actions of second messenger molecules. The two most extensively studied second messengers are Ca2+ and cAMP, each of which...
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Chapter
Modification of Leucyl-tRNA Synthetase by Affinity Labeling and Limited Proteolysis
Aminoacyl-tRNA synthetases catalyses catalyze the transfer of amino acid to its cognate tRNA.
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Chapter
Laser Raman Spectroscopy of Nucleic Acids
Recent applications of the technique of single crystal X-ray diffraction, in combination with structure refinement methods, have revealed architectural details of right- and left-handed DNA helices at atomic r...
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Chapter
Unusual DNA Structures and the Probes Used for their Detection
A veritable explosion in our knowledge of unusual DNA structures has occurred in recent years. Our enhanced knowledge about specific DNA sequences via DNA sequencing and cloning has been a contributory factor....
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Reinvestigation on BNPS-Skatole Cleavage of Tryptophan Polypeptides and the C-Terminal Sequencing of the Cleavage Fragments
In the light of indole chemistry Patchornik and Witkop (1960) have proposed the mechanism of oxidative cleavage of tryptophan polypeptides and dioxyindolylalanine spiro lactone (Dial) as the oxidation product ...
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Chapter and Conference Paper
Regulation of the Rat Insulin II Gene: cis- and trans-acting Factors
Insulin is one of the key peptide hormones in the control of growth and metabolism. The imbalance of insulin action results in diabetes mellitus which has broad impact on the society. Aside from its medical im...
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Catalysis by Isomeric Forms of Covalently Labeled F1-ATPase
When bovine heart mitochondrial F1-ATPase was labeled with low concentrations of NBD-Cl* in the dark in the presence of ATP at pH 7, the NBD-label was almost exclusively attached to a specific tyrosine residue un...
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Chapter and Conference Paper
Regulation of Glucocorticoid Receptor Protein and Gene Expression by Glucocorticoids
Glucocorticoids, members of the highly conserved steroid hormone family in evolution, exert numerous physiological effects on the developmental and adaptational processes of eukaryotic organisms. In general, f...
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Chapter and Conference Paper
Recent Progress on Understanding the Initiation of Translation in the Chloroplasts of Euglena Gracilis
The mechanism of protein biosynthesis in chloroplasts is currently not well understood. However, in general, the process of translation in this organelle is thought to resemble that in prokaryotes more closely...
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Chapter and Conference Paper
Field trial of an inactivated vaccine against hemorrhagic fever with renal syndrome in humans
Hantaan virus was serially passaged in the brains of suckling rats. Vaccine inactivated with formalin was prepared from the suckling rat brains and the antigenic potency of the vaccine was determined by enzyme...
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Chapter and Conference Paper
Evidence for three Phytochromes in Avena
Shortly after the identification of phytochrome as a chromoprotein, Butler and Lane (1965) raised the question of whether the ‘bulk’ phytochrome detected in etiolated seedlings was physiologically functional, ...
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Chapter and Conference Paper
Telomere Replication in Saccharomyces cerevisiae
Conventional DNA polymerases cannot start DNA synthesis de novo and can synthesize DNA only in the 5′ to 3′ direction. It was pointed out by Watson (1972) that these properties make it impossible for conventional...
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Chapter
New Methodologies in Heparin Structure Analysis and the Generation of LMW Heparins
This chapter examines the methods used to prepare heparin, low molecular weight (LMW) heparins, and immobilized heparins. These heparins and heparin derivatives are polydisperse heterogeneous mixtures that req...
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Chapter
Developmental changes of FABP concentration, expression, and intracellular distribution in locust flight muscle
M-FABP from flight muscle of the locust, Schistocerca gregaria, is similar to mammalian H-FABP in its physical characteristics and amino acid sequence. We have studied developmental changes using ELISA, Northern ...
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Chapter
Structure and Function of Escherichia Coli Ribosomal Protein L7/L12: Effect of Cross-Links and Deletions
Ribosomal protein L7/L12 of E. coli is the most extensively investigated representative of the small, four-copy, dimeric acidic proteins that are found in large ribosomal subunits of all organisms. First characte...
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Analysis of Isoelectric Variants of Carbohydrate Binding Protein 35
Carbohydrate Binding Protein 35 (CBP35) is a galactose-specific lectin belonging to the L-30 group of the S-type family of animal lectins (Wang et al. 1991; Anderson and Wang 1992). The polypeptide chain (Mr ~ 35...
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Chapter
Microsomal Amidases and Carboxylesterases
Deacylation is an important biological reaction which affects the efficacy and toxicity of chemotherapeutic amides and the carcinogenicity of aryl and heterocyclic amides. It is catalyzed by amidases, a group ...
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Chapter and Conference Paper
Human Chorionic Gonadotropin: Progress in Determining Its Tertiary Structure
In humans, maintenance of pregnancy requires extending the lifetime of the corpus luteum, which produces essential steroids. The signal for this maintenance is provided by human chorionic gonadotropin (hCG), a di...
