Abstract
M-FABP from flight muscle of the locust, Schistocerca gregaria, is similar to mammalian H-FABP in its physical characteristics and amino acid sequence. We have studied developmental changes using ELISA, Northern Blotting, and EM/immuno-gold techniques. M-FABP is found in cytoplasm and nuclei, but not in mitochondria. It is the most abundant soluble muscle protein in fully developed adult locusts, comprising 18% of the total cytosolic protein. However, no FABP is detectable at the beginning of the adult stage. Its concentration rises dramatically during the next 10 days, after which it reaches its maximal value. Expression apparently is turned on after adult ecdysis and continues for 10 days; thereafter, FABP mRNA diminishes and reaches a constant, but low level, probably needed to maintain the current FABP level. From a series of experiments employing metamorphosis-controlling hormones and antihormones it is evident that the induction of FABP expression is directly linked to metamorphosis.
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Abbreviations
- ELISA:
-
Enzyme Linked Immuno Sorbent Assay
- FABP:
-
Fatty Acid-Binding Protein
- H-FABP:
-
mammalian Heart Fatty Acid-Binding Protein
- M-FABP:
-
locust flight Muscle Fatty Acid-Binding Protein
References
Veerkamp JH, Peeters RA, Maatman RGHJ: Structural and functional features of different types of cytoplasmic fatty acid-binding proteins. Biochim Biophys Acta 1081: 1–24, 1991
Matarcse V, Stone RL, Waggoner DW, Bernlohr DA: Intracellular fatty acid trafficking and the role of cytosolic lipid binding proteins. Prog Lipid Res 28: 245–272, 1989
Kaikaus RM, Bass NM, Ockner RK: Function of fatty acid binding proteins. Experientia 46: 617–630, 1990
Peeters RA, in’t Groen MA, Veerkamp JH: The fatty acid-binding protein form human skeletal muscle. Arch Biochem Biophys 274: 556–563, 1989
Kaufman M, Simoncau JA, Veerkamp JH, Pette D: Electrostim-ulation-induced increases in fatty acid binding protein and myoglobin in rat fast-twitch muscle and comparison with tissue levels in heart. FEBS Lett 245: 181–184, 1989
Beenakkers AM Th, van der Horst DJ, van Marrewijk WJA: Insect flight muscle metabolism. Insect Biochem 14: 243–260, 1984
Haunerland NH, Chisholm JM: Fatty acid binding protein in flight muscle of the locust, Schistocerca gregaria. Biochim Biophys Acta 1047: 233–238, 1990
Price HM, Ryan RO, Haunerland NH: Primary structure of locust flight muscle fatty acid binding protein. Arch Biochem Biophys 297: 285–290, 1992
Chen X, Wang Z, Haunerland NH: Flight muscle FABP expression in juvenile and adult forms of the desert locust, Schistocerca gregaria. Insect Biochem Mol Biol, 23: 337–343, 1992
Haunerland NH, Andolfatto P, Chisholm JM, Wang Z, Chen X: Fatty acid binding protein in locust flight muscle. Developmental changes of expression, concentration, and intracellular distribution. Eur J Biochem, 210: 1045–1051, 1992
Wang Z, Haunerland NH: Ultrastructural study of storage protein granules in fat body of the corn earworm, Heliothis zea. J Insect Physiol 37: 353–363, 1991
Crabtree B, Newsholme EA: Comparative aspects of fuel utilization and metabolism by muscle. In: PNR Usherwood (ed.) Insect Muscle, Academic Press, London, 1975, pp 405–491
Fournier NC, Rahim M: Control of energy production in the heart: a new function for fatty acid binding protein. Biochemistry 24: 2387–2396, 1985
Rembold H: Secondary plant products in insect control, with special reference to the azadirachtins. In: W Engels (ed.) Advances in invertebrate reproduction, Vol 3, Elsevier Science Publishers, Amsterdam, 1984, pp 481–491
Cotton G, Anstee JH: A structural and biochemical study on the effects of methoprene on flight muscle development in Locusta migratoria L. J Insect Physiol 36: 959–969, 1990
Bowers WS: Antihormones. In: GA Kerkut, LI Gilbert (eds) Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol 8, Pergamon, Oxford, 1985, pp 551–564
Börchers T, Unterberg C, Rüdel H, Robenek H, Spener F: Subcellular distribution of cardiac fatty acid-binding protein in bovine heart muscle and quantitation with an enzyme linked immunosorbent assay. Biochim Biophys Acta 1002: 54–61, 1989
Unterberg C, Börchers T, Højrup P, Roepstorff P, Knudsen J, Spener F: Cardiac fatty acid binding proteins. Isolation and characterisation of the mitochondrial fatty acid-binding protein and its structural relationship with the cytosolic isoforms. J Biol Chem 265:16255–16261,1990
Brosemer RW, Vogell W, Bücher T: Morphologische and enzyma-tische Muster bei der Entwicklung indirekter Flugmuskeln von Locusta migratoria. Biochem Z 338: 854–910, 1963
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© 1993 Springer Science+Business Media Dordrecht
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Haunerland, N.H., Chen, X., Andolfatto, P., Chisholm, J.M., Wang, Z. (1993). Developmental changes of FABP concentration, expression, and intracellular distribution in locust flight muscle. In: Glatz, J.F.C., van der Vusse, G.J. (eds) Cellular Fatty Acid-Binding Proteins II. Developments in Molecular and Cellular Biochemistry, vol 10. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3096-1_20
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DOI: https://doi.org/10.1007/978-1-4615-3096-1_20
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