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Changes of collagen type III and decorin in paraurethral connective tissue from women with stress urinary incontinence and prolapse

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  1. Article

    Open Access

    A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends

    Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction s...

    Fanli Zeng, Zhimin Hao, Pan Li, Yanan Meng, **gao Dong, Yibin Lin in BMC Biotechnology (2017)

  2. Article

    Open Access

    AFEAP cloning: a precise and efficient method for large DNA sequence assembly

    Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembl...

    Fanli Zeng, **** Zang, Suhua Zhang, Zhimin Hao, **gao Dong in BMC Biotechnology (2017)

  3. Article

    Open Access

    Efficient strategy for introducing large and multiple changes in plasmid DNA

    While the QuikChange site-directed mutagenesis method and its later modifications are extremely useful and simple, they suffer from several drawbacks. Here, we propose a new method, named LFEAP mutagenesis (Ligat...

    Fanli Zeng, Suhua Zhang, Zhimin Hao, Shixin Duan, Yanan Meng, Pan Li in Scientific Reports (2018)

  4. Article

    Open Access

    Sesamolin serves as an MYH14 inhibitor to sensitize endometrial cancer to chemotherapy and endocrine therapy via suppressing MYH9/GSK3β/β-catenin signaling

    Endometrial cancer (EC) is one of the most common gynecological cancers. Herein, we aimed to define the role of specific myosin family members in EC because this protein family is involved in the progression o...

    Yibin Lin, **ao Chen, Lin** Lin, Benhua Xu in Cellular & Molecular Biology Letters (2024)