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209 Result(s)
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Article
Characterization of S1 nuclease sensitive site at transcription initiation region ofAttacus ricini rDNA
A single-stranded S1 nuclease hypersensitive site which contains a d(AT) sequence structure located in the 5′-non transcription spacer of silkworm A.ricini ribosomal RNA gene has been reported[1]. Using starved-r...
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Identification and characterization of scaffold-associated region (SAR) of rRNA gene of silkwormAttacus ricini
identify the specific nuclear scaffold-bound DNA sequence in rRNA gene clusters of silkwormAttacus ricini, the detergent-like salt lithium 3′, 5′ diiodosalicylate (LIS) was used for the preparation of nuclear sca...
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Article
AFLP-Based detection of DNA methylation
By using the isoschizomersHpa II andMsp I which display differential sensitivity to cytosine methylation, a modified amplified fragment length polymorphism (AFLP) technique has been developed to investigate DNA m...
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Article
AFLP-derived SCARs facilitate construction of a 1.1 Mb sequence-ready map of a region that spans the Vf locus in the apple genome
The availability of high-density anchored markers is a prerequisite for reliable construction of a deep coverage BAC contig, which leads to creation of a sequence-ready map in the target chromosomal region. Un...
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Article
AFLP analysis of genetic variability in New Guinea impatiens
New Guinea impatiens (Impatiens hawkeri) is an economically important floral crop, however, little work has been conducted to further our understanding of the genetics of this crop. In this study, we used amplif...
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Article
Effects of cerium on nitrogen metabolism of peach plantlet in vitro
The effect of CeCl3 on the rooting and nitrogen metabolism of the peach plantlet in vitro was studied when the CeCl3 was added to the rooted medium. The results showed that the rooting rate and the fresh weight o...
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Article
Develo** gene-tagged molecular markers for functional analysis of starch-synthesizing genes in rice (Oryza sativa L.)
The granule-bound starch synthase(GBSS), starch branching enzymes 1 (SBE1)and 3 (SBE3) are major enzymes involved in starch biosynthesis in rice endosperm. Available sequences of Sbe1 and Sbe3 genes encoding corr...
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Article
DNA-methylation alterations and exchanges during in vitro cellular differentiation in rose (Rosa hybrida L.)
DNA-methylation profiles of leaf tissues of Rosa hybrida cv. Carefree Beauty collected from in vivo-grown greenhouse plants, in vitro-grown proliferating shoots at different passages, regenerants of embryogenic c...
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Article
Effect of La(III) on the growth and aging of root of loquat plantlet in vitro
The effect of lanthanum on the adventitious root growth, activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), O2 production rate, MDA content, and the relative plasma membrane permeabil...
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Article
Delimiting a rice wide-compatibility gene S 5 n to a 50 kb region
Wide-compatibility (WC) is one of the most important traits in rice, which can overcome the fertility barrier in the indica/japonica hybrids, and hence to make it possible to utilize the higher yield potential of...
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Article
Genome-wide isolation of resistance gene analogs in maize (Zea mays L.)
Conserved domains or motifs shared by most known resistance (R) genes have been extensively exploited to identify unknown R-gene analogs (RGAs). In an attempt to isolate all potential RGAs from the maize genome, ...
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Article
Functional analysis of starch-synthesis genes in determining rice eating and cooking qualities
Apparent amylose content (AAC), gel consistency (GC), and gelatinization temperature (GT) are recognized as the most important determinants of rice eating and cooking qualities. The contributions of major star...
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Article
Construction of two Lolium perenne BAC libraries and identification of BACs containing candidate genes for disease resistance and forage quality
Two BAC libraries were constructed for the forage and turf grass species Lolium perenne L. The libraries consisted of 98,304 and 101,376 BAC clones for L. perenne genotypes LTS18 and NV#20F1-30, respectively. The...
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Article
Correlation between haplotype of apolipoprotein B gene and natural longevity persons in Uygur Nationality
This paper investigated the correlation between polymorphisms and haplotypes in the apolipoprotein B (apoB) gene (SP-I/D, Xbal-RFLP, VNTR) and natural longevity persons among the Uygur people in **njiang. For thi...
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Article
Growth Plate Zonal Microarray Analysis Shows Upregulation of Extracellular Matrix Genes and Downregulation of Metalloproteinases and Cathepsins following Irradiation
Although the growth plate matrix area fraction increases after irradiation, extracellular matrix (ECM) gene expression in this context has not been studied. The hypothesis was that normally expressed ECM genes...
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Article
Map** of genome-wide resistance gene analogs (RGAs) in maize (Zea mays L.)
Isolation and map** of genome-wide resistance (R) gene analogs (RGAs) is of importance in identifying candidate(s) for a particular resistance gene/QTL. Here we reported our result in map** totally 228 genome...
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Article
Open AccessOptimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics
In proteomic analysis, MS/MS spectra acquired by mass spectrometer are assigned to peptides by database searching algorithms such as SEQUEST. The assignations of peptides to MS/MS spectra by SEQUEST searching ...
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Article
A study of absorption characteristics of chromophoric dissolved organic matter and particles in Lake Taihu, China
Absorptions by non-phytoplankton particles and phytoplankton, and chromophoric dissolved organic matter (CDOM) were measured at 50 sites in large, shallow, Lake Taihu in winter and summer 2006 to study their s...
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Article
Discovery of a new fragrance allele and the development of functional markers for the breeding of fragrant rice varieties
The recessive fgr gene on chromosome 8 is associated with rice fragrance. It has been reported that this gene is a non-functional badh2 allele and that the functional Badh2 allele encoding putative betaine aldehy...
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Article
Identification and fine-map** of a major QTL conferring resistance against head smut in maize
Head smut is one of the most devastating diseases in maize, causing severe yield loss worldwide. Here we report identification and fine-map** of a major quantitative trait locus (QTL) conferring resistance t...