8 Result(s)
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Article
Harold Marcotte
Biotechnology
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Article
Clostridium difficile, the Difficult “Kloster” Fuelled by Antibiotics
Clostridium difficile is normally present in low numbers in a healthy adult gastro-intestinal tract (GIT). Drastic changes in the microbial population, e.g., dysbiosis caused by extensive treatment with antibioti...
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Open AccessLactobacillus delivery of bioactive interleukin-22
Interleukin-22 (IL-22) plays a prominent role in epithelial regeneration and dampening of chronic inflammatory responses by protecting intestinal stem cells from immune-mediated tissue damage. IL-22 has a cons...
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Open AccessIn situgastrointestinal protection against anthrax edema toxin by single-chain antibody fragment producing lactobacilli
Anthrax is caused by the bacterium Bacillus anthracis and is regarded as one of the most prominent bioterrorism threats. Anthrax toxicity is induced by the tripartite toxin complex, composed of the receptor-bindi...
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Screening and Evaluation of Human Intestinal Lactobacilli for the Development of Novel Gastrointestinal Probiotics
The aim of this study was to screen intestinal lactobacilli strains for their advantageous properties to select those that could be used for the development of novel gastrointestinal probiotics. Ninety-three i...
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Open AccessLactobacillli expressing llama VHH fragments neutralise Lactococcusphages
Bacteriophages infecting lactic acid bacteria (LAB) are widely acknowledged as the main cause of milk fermentation failures. In this study, we describe the surface-expression as well as the secretion of two fu...
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Therapeutic effect of llama derived VHH fragments against Streptococcus mutans on the development of dental caries
Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptoco...
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Passive immunization by lactobacilli expressing single-chain antibodies against Streptococcus mutans
We compared the anticariogenic effect of lactobacilli expressing a single-chain antibody (scFv) against the SA I/II adhesin of Streptococcus mutans under the control of an inducible or a constitutive promoter. Bo...
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In situ delivery of passive immunity by lactobacilli producing single-chain antibodies
Lactobacilli have previously been used to deliver vaccine components for active immunization in vivo. Vectors encoding a single-chain Fv (scFv) antibody fragment, which recognizes the streptococcal antigen I/II (...
