23 Result(s)
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Article
David C. Taylor
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Identification, duplication, evolution and expression analyses of caleosins in Brassica plants and Arabidopsis subspecies
Caleosins are a class of Ca2+ binding proteins that appear to be ubiquitous in plants. Some of the main proteins embedded in the lipid monolayer of lipid droplets, caleosins, play critical roles in the degradatio...
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Open AccessDedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production
Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for...
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Open AccessTriacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2
The Arabidopsis thaliana dgat1 mutant, AS11, has an oil content which is decreased by 30%, and a strongly increased ratio of 18:3/20:1, compared to wild type. Despite lacking a functional DGAT1, AS11 still manage...
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Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri
Lesquerella fendleri (commonly known as “Fendler’s bladderpod” or “yellowtop”) is a member of the Brassicaceae and is an important seed oil-producing plant. The lipid profile of L. fendleri seed indicates potenti...
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Increase in nervonic acid content in transformed yeast and transgenic plants by introduction of a Lunaria annua L. 3-ketoacyl-CoA synthase (KCS) gene
Nervonic acid is a Very Long-Chain Monounsaturated Fatty Acid (VLCMFA), 24:1 Δ15 (cis-tetracos-15-enoic acid) found in the seed oils of Lunaria annua, borage, hemp, Acer (Purpleblow maple) and Tropaeolum speciosu...
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Open AccessTransgenic increases in seed oil content are associated with the differential expression of novel Brassica-specific transcripts
Seed oil accumulates primarily as triacylglycerol (TAG). While the biochemical pathway for TAG biosynthesis is known, its regulation remains unclear. Previous research identified microsomal diacylglycerol acyl...
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Hairpin-RNA mediated silencing of endogenous FAD2 gene combined with heterologous expression of Crambe abyssinica FAE gene causes an increase in the level of erucic acid in transgenic Brassica carinata seeds
The 3′-UTR of the FAD2 gene from Brassica carinata was cloned by PCR and used to prepare an intron-spliced hairpin RNA (ihpRNA) construct. Compared to that of the wild type (WT) background, this construct, when e...
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Production of 22:2Δ5,Δ13 and 20:1Δ5 in Brassica carinata and soybean breeding lines via introduction of Limnanthes genes
Seed oils of meadowfoam (Limnanthes douglasii, L. alba) contain very long-chain fatty acids of strategic importance for a number of industrial applications. These include the monoene 20 1Δ5 and the diene 22:2Δ5,Δ...
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Molecular and biochemical characterization of an aminoalcoholphosphotransferase (AAPT1) from Brassica napus: effects of low temperature and abscisic acid treatments on AAPT expression in Arabidopsis plants and effects of over-expression of BnAAPT1 in transgenic Arabidopsis
Aminoalcoholphosphotransferases (AAPT, EC 2.7.8.1 and EC 2.7.8.2) catalyze the transfer of CDP-aminoalcohols to sn-1, 2 diacylglycerol (DAG) to form phosphatidylaminoalcohols with the release of CMP. The Brassica...
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The concept of mental health in children
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Whatever happened to “Child and Adolescent Psychiatry”?
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Field testing of transgenic rapeseed cv. Hero transformed with a yeast sn-2 acyltransferase results in increased oil content, erucic acid content and seed yield
A major goal of our research is to produce, by genetic manipulation, Brassica napus L. cultivars with higher levels of 22:1 in their seed oil than in present Canadian HEA cultivars developed through traditional b...
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Effects of antisense repression of an Arabidopsis thaliana pyruvate dehydrogenase kinase cDNA on plant development⋆
Pyruvate dehydrogenase kinase (PDHK), a negative regulator of the mitochondrial pyruvate dehydrogenase (PDH) complex (mtPDC), plays a pivotal role in controlling mtPDC activity, and hence, the TCA cycle and ce...
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Intraoperative Intraarterial Urokinase in Early Postoperative Stroke following Carotid Endarterectomy: A Useful Adjunct
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Predictive Ability of Duplex Ultrasonography for Internal Carotid Artery Stenosis of 70%-99%: A Comparative Study
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Book reviews
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Cloning of a cDNA encoding the 21.2 kDa oleosin isoform from Arabidopsis thaliana and a study of its expression in a mutant defective in diacylglycerol acyltransferase activity
A full-length cDNA clone (pA23) of 832 bp encoding an oleosin from Arabidopsis thaliana was isolated by differential screening of a silique-specific cDNA library with probes prepared from poly(A)+ RNA isolated fr...
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Stereospecific analysis and mass spectrometry of triacylglycerols fromarabidopsis thaliana (L.) heynh. columbia seed
Arabidopsis thaliana continues to be an excellent model organism for studying plant molecular genetics and biochemistry. In particular, the generation and analysis of mutant lines has fac...
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Stereospecific analyses of seed triacylglycerols from high-erucic acid brassicaceae: Detection of erucic acid at thesn-2 position inBrassica oleracea L. Genotypes
Stereospecific analyses of triacylglycerols from selected high-erucic acid breeding lines or cultivars ofBrassica napus L. andB. oleracea L. have been performed. Initial lipase screening revealed that while allB....
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Formation of trierucoylglycerol (trierucin) from 1,2-Dierucoylglycerol by a homogenate of microspore-derived embryos ofBrassica napus L
Homogenates of microspore-derived embryos of rape (Brassica napus L.) incubated with [1-14C]erucoyl-CoA and 1,2-dierucoylglycerol are able to assemble trierucoyl-glycerol (trierucin). In addition, radioactive tri...
