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Article
Reinvigorating natural product combinatorial biosynthesis with synthetic biology
Natural products continue to play a pivotal role in drug-discovery efforts and in the understanding of human health. The ability to extend nature's chemistry through combinatorial biosynthesis—altering functio...
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Article
Develo** Streptomyces venezuelae as a cell factory for the production of small molecules used in drug discovery
The heterologous expression of biosynthetic pathways is an indispensable tool for drug discovery and development from natural products. Streptomyces venezuelae is a promising heterologous host as it offers severa...
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Article
Structural modification of herboxidiene by substrate-flexible cytochrome P450 and glycosyltransferase
Herboxidiene is a natural product produced by Streptomyces chromofuscus exhibiting herbicidal activity as well as antitumor properties. Using different substrate-flexible cytochrome P450s and glycosyltransferase,...
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Article
Relationship between dyslipidemia and albuminuria in prediabetic adults: The Korea National Health and Nutrition Examination Survey 2011–2012
This study aimed to estimate the relationship between various lipid abnormalities and albuminuria in Korean prediabetic adults. Data obtained from the Korea National Health and Nutrition Examination Survey (KN...
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Article
Characterization of negative regulatory genes for the biosynthesis of rapamycin in Streptomyces rapamycinicus and its application for improved production
Sequence analysis of the rapamycin biosynthetic gene cluster in Streptomyces rapamycinicus ATCC 29253 identified several putative regulatory genes. The deduced product of rapY, rapR, and rapS showed high sequence...
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Article
Characterization and engineering of the ethylmalonyl-CoA pathway towards the improved heterologous production of polyketides in Streptomyces venezuelae
Streptomyces venezuelae has an inherent advantage as a heterologous host for polyketide production due to its fast rate of growth that cannot be endowed easily through metabolic engineeri...
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Article
Open AccessEnzymatic synthesis of epothilone A glycosides
Epothilones are extremely cytotoxic chemotherapeutic agents with epoxide, thiazole, and ketone groups that share equipotent kinetic similarity with taxol. The in vitro glycosylation catalyzed by uridine diphos...
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Article
Achievements and impacts of glycosylation reactions involved in natural product biosynthesis in prokaryotes
Bioactive natural products, such as polyketides, flavonoids, glycopeptides, and aminoglycosides, have been used as therapeutic agents. Many of them contain structurally diverse sugar moieties attached to the a...
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Article
Re-engineering of genetic circuit for 2-deoxystreptamine (2-DOS) biosynthesis in Escherichia coli BL21 (DE3)
Various approaches for monocistronic constructions of genetic circuits have been designed for metabolite production but there has been no attempt to apply such methodology for aminoglycosides biosynthesis. Her...
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Article
Enhancement of Long-Chain Fatty Acid Production in Escherichia coli by Coexpressing Genes, Including fabF, Involved in the Elongation Cycle of Fatty Acid Biosynthesis
The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathwa...
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Article
Glycosylation of various flavonoids by recombinant oleandomycin glycosyltransferase from Streptomyces antibioticus in batch and repeated batch modes
An oleandomycin glycosyltransferase (OleD GT) gene from Streptomyces antibioticus was functionally expressed in Escherichia coli BL21 (DE3) with various molecular chaperones. The purified recombinant OleD GT cata...
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Article
Functional analysis of ABC transporter genes pdmR1 and pdmR2 in Actinomadura hibisca P-1752 and enhancement of pradimicin production
Two putative ABC transporter genes pdmR1 and pdmR2 are found in the biosynthetic gene cluster of pradimicin. pdmR1 showed a hydrophilic profile with similarity to the daunorubicin-resistant ABC transporter ATP-bi...
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Article
Engineered biosynthesis of glycosylated derivatives of narbomycin and evaluation of their antibacterial activities
A 14-membered macrolide antibiotic narbomycin produced from Streptomyces venezuelae ATCC 15439 is composed of polyketide macrolactone ring and D-desosamine as a deoxysugar moiety, which acts as an important deter...
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Article
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation
Investigations into kanamycin biosynthesis and identification of new pathway intermediates surprisingly point to the substrate specificity of two glycosyltransferases as controlling flux into parallel pathways...
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Article
A combined approach of classical mutagenesis and rational metabolic engineering improves rapamycin biosynthesis and provides insights into methylmalonyl-CoA precursor supply pathway in Streptomyces hygroscopicus ATCC 29253
Rapamycin is a macrocyclic polyketide with immunosuppressive, antifungal, and anticancer activity produced by Streptomyces hygroscopicus ATCC 29253. Rapamycin production by a mutant strain (UV2-2) induced by ultr...
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Article
Generation of reduced macrolide analogs by regio-specific biotransformation
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Article
Biosynthesis of rapamycin and its regulation: past achievements and recent progress
Rapamycin and its analogs are clinically important macrolide compounds produced by Streptomyces hygroscopicus. They exhibit antifungal, immunosuppressive, antitumor, neuroprotective and antiaging activities. The ...
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Article
Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides
Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides. To pro...
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Article
Genetic engineering of macrolide biosynthesis: past advances, current state, and future prospects
Polyketides comprise one of the major families of natural products. They are found in a wide variety of bacteria, fungi, and plants and include a large number of medically important compounds. Polyketides are ...
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Article
Enhanced FK506 production in Streptomyces clavuligerus CKD1119 by engineering the supply of methylmalonyl-CoA precursor
FK506 is a 23-membered polyketide macrolide with immunosuppressant activity produced by Streptomyces species. The production of FK506 in S. clavuligerus CKD1119 (KCTC 10561BP) was improved by enhancing the supply...
