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Article
Recombinant feline herpesvirus type 1 expressing Toxoplasma gondii ROP2 antigen inducible protective immunity in cats
In order to investigate whether a recombinant viral vaccine composed of a feline herpesvirus type 1 (FHV1) vector and an immunogenic antigen of Toxoplasma gondii can induce a protective immunity in cats, a recom...
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Article
Delivery of Neospora caninum surface protein, NcSRS2 (Nc-p43), to mouse using recombinant vaccinia virus
In order to develop a vaccine against Neospora caninum in dogs and cattle, we constructed a recombinant vaccinia virus expressing the N. caninum surface protein, NcSRS2 (Nc-p43). Monoclonal antibodies to NcSRS2 ...
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Article
Growth-inhibitory effects of interferon-γ on Neospora caninum in murine macrophages by a nitric oxide mechanism
To clarify the effector pathway of Neospora caninum growth-inhibitory activity induced by interferon-γ (IFN-γ) in murine macrophages we examined the relationship between IFN-γ and nitric oxide (NO). Production o...
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Article
Ultrastructural characteristics of Babesia caballi in equine erythrocytes in vitro
Babesia caballi cultured continuously in equine erythrocytes was examined by transmission electron microscopy. The use of cultured B. caballi permitted examination of a large number of parasitized cells with var...
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Article
Molecular Cloning and Sequence Analysis of the Phosphoprotein (P) Gene of the Lapinized Rinderpest Virus
We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlap** open reading frames of 1521 and 531 nucleo...
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Article
Construction of Canine Herpesvirus Vector Expressing Foreign Genes Using a lacZ-TK Gene Cassette as a Double Selectional Marker
An improved method for constructing canine herpesvirus (CHV) recombinants expressing foreign genes by using the lacZ-TK gene cassette as a double selectional marker was developed. A recombinant CHV carrying th...
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Article
Identification and Characterization of the Feline Herpesvirus Type 1 Glycoprotein C Gene
The feline herpesvirus type 1 (FHV-1) gene encoding glycoprotein C (gC) has been sequenced and identified based on its genomic location and comparative analysis to other alphaherpesvirus gCs, and the expressed...
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Article
Identification and DNA Sequence Analysis of the Marek’s Disease Virus Serotype 2 Genes Homologous to the Thymidine Kinase and UL24 Genes of Herpes Simplex Virus Type 1
The thymidine kinase (TK) gene has been used as a safe and convenient locus for expression of heterologous proteins in some alphaherpesviruses including herpesvirus of turkeys (HVT) antigenically related to Ma...
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Article
Identification and nucleotide sequence of the thymidine Kinase gene of canine herpesvirus
This paper presents the entire nucleotide sequence of the thymidine Kinase (TK) gene of canine herpesvirus (CHV). The gene was located within a 2.1 kbpEcoRV fragment by Southern-blot hybridization with a probe de...
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Article
Induction of platelet-activating factor in mice by intravenous administration of a neutral fraction of bakers' yeast mannan
A neutral subfraction of mannan of bakers' yeast (WNM) was found to show a lethal effect in mice when administered intravenously. Symptoms caused by intravenous (i.v.) administration of WNM resembled those res...
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Article
Preliminary comparisons of the biological properties of two strains of feline immunodeficiency virus (FIV) isolated in Japan with FIV Petaluma strain isolated in the United States
Two strains of feline immunodeficiency virus (FIV) were isolated directly from peripheral blood mononuclear cells (PBMCs) of Japanese domestic cats or indirectly from PBMCs of specific pathogen free (SPF) cats...
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Article
Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus
Interleukin-2 dependent feline T-lymphoblastoid cells designated as MYA-1 cells were established. The cells were free from exogenous retroviruses and sensitive for replication of feline immunodeficiency virus ...