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Chapter and Conference Paper
An Overview of Cytokinin Biosynthesis
A critical element in understanding the physiological role of cytokinins is the knowledge of how plants control their cytokinin levels. Therefore, we need to know the pathway(s) and site(s) of cytokinin synthe...
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Chapter and Conference Paper
Xylem-Translocated Cytokinin: Metabolism and Function
The concept that cytokinin synthesized in the root moves via the xylem to the shoot to regulate some aspects of development, and especially leaf senescence, is often proposed [2]. However, studies of the distr...
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Article
The effect of auxin concentration on cytokinin stability and metabolism
The stability of [3H]zeatin riboside supplied to freshly excised tobacco pith explants was found to be inversely related to α-naphthaleneacetic acid concentration in the incubation medium. At higher concentration...
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Article
Identification of cytokinin glucosides in a seaweed extract
Zeatin-O-glucoside, its dihydro derivative, and dihydrozeatin riboside-O-glucoside have been identified as the main cytokinin-O-glucosides in Seasol, a commercial preparation from Tasmanian giant bull kelp mar...
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Article
Indole-3-acetic acid determination in cultured crown-gall and genetic tumour tissues by gas chromatography-mass spectrometry
Indole-3-acetic acid (IAA) was identified and quantified in three cultured tumour lines, which included crown-gall tissue of Datura (25.4 ng/g fresh wt.) and two genetic tumour lines of tobacco (4.6 and 8.0 ng/g ...
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Chapter
GC-MS Methods for Cytokinins and Metabolites
Cytokinin is a generic term proposed to define both naturally occurring and synthetic compounds that induce cell division in plant tissue cultures (e.g., tobacco stem pith, soybean cotyledonary callus and carr...
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Article
Cytokinins in maturing and germinatingLupinus luteus L. seeds
3H-labelled zeatin riboside (ZR) was applied to pod walls of intactLupinus luteus L. plants. Metabolites present in mature, dry seeds were zeatin nucleotide (ZNT), zeatin riboside (ZR) and zeatin...
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Article
Cytokinin biosynthesis in plant tumour tissues
A range of endogenous cytokinins have been identified inDatura crown-gall tissue by GC-MS. Incorporation of [3H]adenine into zeatin riboside, zeatin and its nucleotide(s) is also shown. Metabolism studies usingci...
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Article
The stability and biological activity of cytokinin metabolites in soybean callus tissue
The activity, uptake and metabolism of cytokinin metabolites was determined in soybean (Glycine max (L.) Merr.) callus tissue. The following activity sequence was established: zeatin riboside (ZR)>zeatin (Z)>O-gl...
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Article
Cytokinins in transfer RNA of normal and crown-gall tissue of Vinca rosea
cis-Zeatin riboside was identified in transfer-RNA hydrolysates from both normal and crown-gall tissue of Vinca rosea L. The trans-isomer was associated exclusively with the crowngall transfer-RNA. The importanc...
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Article
Regulators of cell division in plant tissues XXIX. The activities of cytokinin glucosides and alanine conjugates in cytokinin bioassays
In a number of cytokinin bioassays, the activities of the following compounds were compared: 3-, 7-, and 9-glucosides of 6-benzylaminopurine (BAP); 7- and 9-glucosides of zeatin; O-glucosides of zeatin, dihydr...
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Article
Cytokinin biochemistry in relation to leaf senescence I. The metabolism of 6-benzylaminopurine and zeatin in oat leaf segments
The metabolism of zeatin and that of 6-benzylaminopurine (BAP) have been compared in oat leaf segments in relation to the markedly differing ability of these cytokinins to retard senescence of such segments. F...
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Article
Cytokinin biosynthesis in crown-gall tissue ofVinca rosea
When care was taken to minimise the effects of phosphatase activity during extraction ofVinca rosea crown-gall tumour tissue, a large proportion of extractable cytolinin activity was present in the nucleotide fra...
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Chapter and Conference Paper
Cytokinin Biosynthesis and Metabolism inVinca rosea Crown Gall Tissue
Progress in the understanding of the mechanisms and controls of plant hormone biosynthesis and metabolism has to a large extent been limited by the availability of suitable systems for study. Whilst the ultima...
