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Article
Sperm antigen MSH27 participates in sperm-egg membrane fusion
A monoclonal antibody (mAb), MSH27 has been selected from an mAb library which has been prepared with separated mouse sperm heads as antigens. Three aspects of evidence indicated that the MSH27 antigen was inv...
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Article
The giant panda (Ailuropoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg
The giant panda skeletal muscle cells, uterus epithelial cells and mammary gland cells from an adult individual were cultured and used as nucleus donor for the construction of intenpecies embryos by transferri...
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Article
Nuclear transfer using nonquiescent adult fibroblasts from a bovine ear
The natural reproduction of mammal is sexual reproduction, which needs fertilization involving sperm and oocyte. Nuclear transfer provided an asexual reproduction method for mammal. Donor cells used in previou...
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Chapter and Conference Paper
Vision-Based Registration Using 3-D Fiducial for Augmented Reality
One of the key issues in the realization of Augmented Reality is the registration problem. Synthetically, vision-based registration can offer superior solutions. In several existing registration methods, 2-D o...
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Chapter
Use of Antioxidants in Cardiovascular Medicine
Interest in the use of antioxidants in the prevention and treatment of cardiovascular disease has mushroomed in the past decade. This is evident from extensive advertisement in medical and non-medical media to...
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Article
Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda
A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blas...
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Article
Valuing ecological functions of biodiversity in Changbaishan Mountain Biosphere Reserve in Northeast China
Conservation of biodiversity can generate considerable indirect economic value and this is being increasingly recognized in China. For a forest ecosystem type of a nature reserve, the most important of its val...
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Article
Advances in interspecific pregnancy
Interspecific pregnancy in which the conceptus and female carrying the pregnancy are of different species is a key step to interspecific cloning. Cloning endangered animals by interspecific pregnancy is such a...
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Article
Effects of ubiquitin-proteasome pathway on mouse sperm capacitation, acrosome reaction and in vitro fertilization
Chlortetracycline (CTC) fluorescence patterns were used to study changes in the patterns B and AR of mouse sperm after incubation with reagents that would block the UPP. They were the monoclonal antibody again...
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Article
Serial nuclear transfer improves the development of interspecies reconstructed giant panda (Aluropoda melanoleuca) embryos
Interspecies somatic nuclear transfer (NT) may provide a new approach for preservation of the endangered rare species. Previous interspecies cloning studies have shown that a nucleus from a quiescent somatic c...
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Article
MAPK regulates cell cycle progression in pig oocytes and fertilized eggs
Oocytes collected from prepubertal gilt ovaries were matured in vitro (IVM), and fertilized in vitro (IVF) or electrically activated. Phosphorylation of mitogenactivated protein kinase (MAPK) was detected with SD...
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Article
Roles of MAP kinase signaling pathway in oocyte meiosis
Mitogen-activated protein kinase (MAPK) is a family of Ser/Thr protein kinases expressed widely in eukaryotic cells. MAPK is activated by a cascade of protein kinase phosphorylation and plays pivotal roles in ...
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Article
Embryonic stem cell as nuclear donor could promote in vitro development of the heterogeneous reconstructed embryo
The nucleus of a somatic cell could be dedifferentiated and reprogrammed in an enucleated heterogeneous oocyte. Some reconstructed oocytes could develop into blastocysts in vitro, and a few could develop into ter...
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Article
Global Trade in GM Food and the Cartagena Protocol on Biosafety: Consequences for China
The UN Cartagena Protocol onBiosafety adopted in Montreal, 29 January, 2000and opened for signature in Nairobi, 15–26 May,2000 will exert a profound effect oninternational trade in genetically modifiedorganism...
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Chapter
Identification, Regulation and Function of Lox-1, a Novel Receptor for Ox-Ldl
Oxidatively modified low-density lipoprotein (ox-LDL) causes endothelial activa-tion, dysfunction and injury, which are considered primary steps in atherogenesis. Recently, a novel lectin-like receptor for ox-...
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Article
Expression of LIF, VEGF, CD57 and CD68 after the transfer of rat embryos to mouse uteri
The high failure rate of interspecific pregnancy is a major obstacle to the successful interspecific cloning of mammals. To investigate the reasons for the failure of interspecfic pregnancy between rats and mi...
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Article
Somatic cell bovine cloning: Effect of donor cell and recipients
Adult somatic cell nuclear transfer was conducted by using cultured ear fibroblast cells obtained from a Holstein female cow (GN) and a Galoway herd bull (GLV). The percentages of reconstructed eggs developed ...
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Article
Effects of different nuclear recipients on developmental potential of mouse somatic nuclear transfer embryos
In order to investigate the effects of different kinds of nuclear recipients from Kunming (KM) mouse on developmental potential of somatic nuclear transfer embryos, the enucleated M II oocytes, enucleated zygo...
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Article
Replacement of inner cell mass in mouse
The intraor inter-strain reconstituted blastocysts were produced by replacing the inner cell mass of Kunming mouse blastocysts with that of Kunming or C57BL/6 mouse strain blastocysts. A total of 192 intra-str...
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Article
Dual roles of progesterone in embryo implantation in mouse
Progesterone (P4) is essential for the development of endometrial receptivity for blastocyst implantation and pregnancy maintenance. Many studies have demonstrated that P4 restrains endometrial tissue breakdown b...
