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Open AccessEctopic expression of a truncated CD40L protein from synthetic post-transcriptionally capped RNA in dendritic cells induces high levels of IL-12 secretion
RNA transfection into dendritic cells (DCs) is widely used to achieve antigen expression as well as to modify DC properties. CD40L is expressed by activated T cells and interacts with CD40 receptors expressed ...
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Article
Improved somatic embryo maturation in loblolly pine by monitoring ABA-responsive gene expression
During loblolly pine zygotic embryo development, increases in mRNAs for three ABA-responsive LEA-like genes coincided with the two developmental stage-specific peaks of endogenous ABA accumulation (Kapik et al. 1...
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Article
Mutations in GLIS3 are responsible for a rare syndrome with neonatal diabetes mellitus and congenital hypothyroidism
We recently described a new neonatal diabetes syndrome associated with congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys1. Here, we show that this syndrome results from mutat...
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Article
Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum
Widespread use of antimalarial agents can profoundly influence the evolution of the human malaria parasite Plasmodium falciparum. Recent selective sweeps for drug-resistant genotypes may have restricted the genet...
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Article
Inheritance and expression of multiple disease and insect resistance genes in transgenic rice
2–3 anti-fungal disease genes are coinserted with hygromycin phosphotransferase in the same vector. Two insecticidal genes and PPT acetyl transferase genes are placed in another one. The vectors are co-deliver...
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Gene Expression Differences Between Zygotic and Somatic Embryos Monitored by Differential Display and cDNA Array: A Potential Tool to Improve Loblolly Pine Somatic Embryo Quality
A continued supply of low cost, high quality fiber is essential for the future success of the U.S. forest products industry. With the worldwide demand for paper expected to increase nearly 50% by the year 2010...