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Chapter
Role of the E2 core in the dominant mechanisms of regulatory control of mammalian pyruvate dehydrogenase complex
The mammalian pyruvate dehydrogenase complex (PDC) is a large assembly composed of six components with nine distinct subunits. Four of these components execute the overall reaction through a series of steps li...
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Chapter
Ceramide Synthase and Ceramidases in the Regulation of Sphingoid Base Metabolism
Ceramides and sphingoid bases (such as sphingosine and sphinganine) are not only intermediates of complex sphingolipid biosynthesis and turnover, but also highly bioactive compounds that have been proposed to ...
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Chapter and Conference Paper
Peptide-Secreting Dendrites: New Controls for Neuroendocrine Neurons
Peptidergic neurons are well known to synthesize and package peptides in their perikarya, and transport the peptide-containing dense-cored vesicles along their axons for release at the axon terminals. Immunocy...
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Chapter
Inward Rectifying Potassium Conductance in Barley Aleurone Protoplasts
The mature barley grain consists mainly of embryo and starchy endosperm which is surrounded by three layers of cells, the aleurone. The aleurone cells are activated upon water imbibition. This activation is ma...
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Chapter and Conference Paper
Molecular Approaches to Biochemical Purification: The COP9 Complex Paradigm
Light signals perceived from the photoreceptors are transduced into the nucleus to regulate gene expression and development. The nuclear-localized COP9 protein complex has a central role in modulating light-re...
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Chapter
Membrane Potential and Action Potential Measurements in Whole Cell and Perforated Patch Configurations
The cell membrane is a permeability barrier separating the intracellular, cytoplasmic, space from the extracellular environment. In neurons, as in any other cell type, the intracellular ionic composition diffe...
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Chapter
Detection of Proteolytic Enzymes Using Protein Substrates
Short peptidic substrates are powerful tools for studying and detecting proteases. However, the natural substrates for the majority of proteases are in fact proteins. The larger size of a protein substrate can...
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Chapter
Genetically Modified Mice as Tools for Cancer Research
Cancer research over the past decades has taught us that more than 80% of human cancers are sporadic with no obvious hereditary contribution and that they arise through gene acquisitions or by gene losses. The...
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Chapter
ATP-Dependent Potassium Channels in the Kidney
The application of the patch-clamp technique (NEHER and SAKMANN 1976) to the kidney has led to the discovery of well-defined potassium (K) channels in the apical and basolateral membrane of tubule cells along ...
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Chapter and Conference Paper
Pyridoxal 5-Phosphate and Calcium Channels
The BAY K 8644 -induced influx of 45Calcium into intracellular compartment of artery segments of normal rats was blocked by pyridoxal phosphate (PLP) as well as by the dihydropyridine-sensitive calcium channel an...
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Chapter
Cell Preparation and Enrichment for FCM Analysis and Cell Sorting
Flow cytometry and cell sorting are really tools for answering biological questions. Some of these questions can be answered directly by analyzing cell populations that reside within heterogeneous starting mat...
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Chapter
Novel Calcium/Calmodulin-modulated Proteins
Ca2+/CaM-regulated protein phosphorylation is believed to play a pivotal role in amplifying and diversifying the action of Ca2+-mediated signals (Poovaiah and Reddy 1993). Although Ca2+/CaM-dependent protein phos...
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Chapter and Conference Paper
Regulated expression of non-polymorphic gp49 molecules on mouse natural killer cells
Murine natural killer (NK) cells express inhibitory receptors belonging to the C-type lectin-like (Ly-49, CD94/NKG2) and Ig-superfamily related (gp49) receptors. The murine gp49B receptor displays structural h...
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Chapter
Use of DNA Markers in introgression and isolation of genes associated with durable resistance to rice blast
Rice blast caused by Pyricularia grisea Sacc. (= P. oryzae Cav., teleomorph Magnaporthe grisea Barr.), is one of the most widespread and destructive diseases of rice. Incorporation of blast resistance genes into ...
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Chapter
Genetically Engineered Animal Models: Physiological Studies with Gastrin in Transgenic Mice
The role of gastrin as a regulator of acid secretion has been appreciated for almost a century.(13) For many years it has been clear that while gastrin may act directly on parietal cells, it also acts on enteroch...
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Chapter and Conference Paper
Culture of Porcine Hepatocytes Using Radial Flow Bioreactor System
Porcine primary hepatocytes were cultured in a radial flow bioreactor system to examine the effects of ascorbic acid 2-phosphate (Asc2-P) and dissolved oxygen concentration (DO) on the ammonium metabolism. The...
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Chapter
Mechanics of vimentin intermediate filaments
It is increasingly evident that the cytoskeleton of living cells plays important roles in mechanical and biological functions of the cells. Here we focus on the contribution of intermediate filaments (IFs) to ...
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Chapter
A Computer Visualization Model for the De Novo Sphingolipid Biosynthetic Pathway
The de novo biosynthesis pathway for sphingolipids has thousands of individual components. This creates difficulties for scientists who conduct “sphingolipidomic” analysis of cells and must deal with a very large...
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Chapter
Isolation of nuclear and cytoplasmic proteins from DT40 cell lines
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Chapter
Molecular Chaperones and Cancer Immunotherapy
As one of the most abundant and evolutionally conserved intracellular proteins, heat shock proteins, also known as stress proteins or molecular chaperones, perform critical functions in maintaining cell homeos...