53 Result(s)
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Article
Minghong Gu
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Article
Centromere inactivation in a dicentric rice chromosome during sexual reproduction
T0135 is a variant selected from the progeny of a rice line telotrisomic for the short arm of chromosome 11 (2n+11S·). Fluorescent in situ hybridization (FISH) results indicated that T0135 contained two telocentr...
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Article
Molecular Cytological Characterization of Somatic Variation in Rice Aneuploids
Aneuploids cannot be stably preserved by sexual propagation, though they can maintain their genetic identity by asexual propagation. However, it is possible for somaclonal variation to occur during asexual pro...
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Identification and diversity of functional centromere satellites in the wild rice species Oryza brachyantha
The centromere is a key chromosomal component for sister chromatid cohesion and is the site for kinetochore assembly and spindle fiber attachment, allowing each sister chromatid to faithfully segregate to each...
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Hd-q, a novel allele of Ef7 from a Chinese rice landrace, confers weak photoperiod sensitivity and improves local adaptability and yield potential
Heading date is a major determinant of adaptability and yield potential in rice (Oryza sativa L.) and is influenced by photoperiod. Among chromosome segment substitution lines, the introgression line C63 contains...
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Open AccessGenetic analysis and gene cloning of a triangular hull 1 (tri1) mutant in rice (Oryza sativa L.)
Grain shape and size are two key factors that determine rice yield and quality. In the present study, a rice triangular hull mutant (tri1) was obtained from the progeny of japonica rice variety Taipei 309 treated...
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Article
RNAi-directed down-regulation of RSV results in increased resistance in rice (Oryza sativa L.)
Rice stripe disease (RSD), caused by rice stripe virus (RSV), is a serious disease in temperate rice-growing areas. We have created an RNAi construct containing coat protein gene (CP) and disease specific prot...
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Article
Diversification of the Waxy Gene is Closely Related to Variations in Rice Eating and Cooking Quality
The Wx gene encoding granule-bound starch synthase is responsible for the biosynthesis of amylose, which plays an important role in determining rice eating and cooking quality. However, the functional evaluation ...
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Article
OsAM1 is required for leptotene-zygotene transition in rice
The events occurring at the onset of meiosis have not been fully elucidated. In the present study, OsAM1 was identified in rice (Oryza sativa L.) by map-based cloning. OsAM1, a homolog of Arabidopsis SWI1 and mai...
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Article
Fine map** of a major QTL controlling panicle number in rice
Rice panicle number per plant is a grain yield component that directly influences rice yield. The identification of the genes controlling panicle number will play a vital role in high-yield rice breeding. C307...
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Fine map** and candidate gene analysis of ptgms2-1, the photoperiod-thermo-sensitive genic male sterile gene in rice (Oryza sativa L.)
Photoperiod-thermo-sensitive genic male sterile (PTGMS) rice exhibits a number of desirable traits for hybrid rice production. The cloning genes responsible for PTGMS and those elucidating male sterility mecha...
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Non-homologous chromosome pairing and crossover formation in haploid rice meiosis
While many studies have provided significant insight into homolog pairing during meiosis, information on non-homologous pairing is much less abundant. In the present study, fluorescence in situ hybridization (FIS...
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Article
OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice
Spo11 is a homolog of a subunit of archaebacterial topoisomerase, which catalyzes DNA double-strand breaks and initiates homologous chromosome recombination. In the present study, we silenced the SPO11-1 gene in ...
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Open AccessDevelo** high throughput genotyped chromosome segment substitution lines based on population whole-genome re-sequencing in rice (Oryza sativa L.)
Genetic populations provide the basis for a wide range of genetic and genomic studies and have been widely used in genetic map**, gene discovery and genomics-assisted breeding. Chromosome segment substitutio...
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Development of gene-tagged molecular markers for starch synthesis-related genes in rice
Improving grain quality is an important goal in breeding new elite rice varieties, requiring effective tools for the identification of target genotypes. Molecular marker-aided selection (MAS), combined with co...
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Genetic analysis and gene fine map** of a rolling leaf mutant (rl 11(t) ) in rice (Oryza sativa L.)
The exploration of new genes controlling rice leaf shape is an important foundation for rice functional genomics and plant architecture improvement. In the present study, we identified a rolling leaf mutant from
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Article
Unstable transmission of rice chromosomes without functional centromeric repeats in asexual propagation
During sexual propagation of primary trisomic 8, chromosome 8 breaks in some rice plants, resulting in a telotrisomic (2n+·8S) line. In this study, we observed that the extra short arm of chromosome 8 (·8S) ca...
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Article
Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization
Oryza sativa and O. latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, interspecific hybrids of these species were obtained using the embryo rescue technique. Hybrid panicle traits...
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Generating of rice OsCENH3-GFP transgenic plants and their genetic applications
In order to investigate rice functional centromeres, OsCENH3-GFP chimeric gene was constructed and transformed into the indica rice variety, Zhongxian 3037, mediated by Agrobacturium. The integration of the exoge...
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Genetic analysis and molecular map** of a presenescing leaf gene psl1 in rice (Oryza sativa L.)
A rice psl1 (presenescing leaf) mutant was obtained from a japonica variety Zhonghua 11 via radiation of 60Co-y in M2 generation. Every leaf of the mutant began to wither after it reached the biggest length, whil...
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Molecular map** of two semidwarf genes in an indica rice variety Aitaiyin3 (Oryza sativa L.)
Genetic analysis established that Aitaiyin3, a dwarf rice variety derived from a semidwarf cultivar Taiyin1, carries two recessive semidwarf genes. By using simple sequence repeat (SSR) markers, we mapped the ...
