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Application of Electron Microscopes in Nanotoxicity Assessment

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Nanotoxicity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1894))

Abstract

In this chapter, we highlight the applications of electron microscopes (EMs) in nanotoxicity assessment. EMs can provide detailed information about the size and morphology of nanomaterials (NMs), their localization in cells and tissues, the nano-bio interactions, as well as the ultrastructural changes induced by NMs exposure. Here, we share with the readers how we prepare the tissue sample, and the different types of EMs used among the nanotoxicologists. It is possible to deploy conventional EMs along or in combination with other analytical techniques, such as electron energy loss spectroscopy (EELS), energy dispersive X-ray spectroscopy (EDS or EDX), and TEM-assisted scanning transmission X-ray microscopy (STXM), toward further elemental and chemical characterization. Appropriate images are inserted to illustrate throughout this chapter.

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Authors and Affiliations

  1. CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Bei**g, P. R. China

    Junzhe Zhang & **ao He

  2. Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY, USA

    Michael T. Tseng

Authors
  1. Junzhe Zhang
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  2. **ao He
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  3. Michael T. Tseng
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Corresponding authors

Correspondence to **ao He or Michael T. Tseng .

Editor information

Editors and Affiliations

  1. Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, USA

    Qunwei Zhang

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Zhang, J., He, X., Tseng, M.T. (2019). Application of Electron Microscopes in Nanotoxicity Assessment. In: Zhang, Q. (eds) Nanotoxicity. Methods in Molecular Biology, vol 1894. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8916-4_14

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  • DOI: https://doi.org/10.1007/978-1-4939-8916-4_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8915-7

  • Online ISBN: 978-1-4939-8916-4

  • eBook Packages: Springer Protocols

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