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Dissection of Daytime and Nighttime Thermoresponsive Hypocotyl Elongation in Arabidopsis

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Thermomorphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2795))

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Abstract

Hypocotyl elongation in Arabidopsis is widely utilized as a readout for phytochrome B (phyB) signaling and thermomorphogenesis. Hypocotyl elongation is gated by the circadian clock and, therefore, it occurs at distinct times depending on day length or seasonal cues. In short-day conditions, hypocotyl elongation occurs mainly at the end of nighttime when phyB reverts to the inactive form. In contrast, in long-day conditions, hypocotyl elongation occurs during the daytime when phyB is in the photoactivated form. Warm temperatures can induce hypocotyl growth in both long-day and short-day conditions. However, the corresponding daytime and nighttime temperature responses reflect distinct underpinning mechanisms. Here, we describe assays for dissecting the mechanisms between daytime and nighttime thermoresponsive hypocotyl elongation.

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Acknowledgments

This work was supported by the National Institute of Health grant R01GM087388 to M.C.

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Authors and Affiliations

  1. Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, CA, USA

    De Fan & Meng Chen

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  1. De Fan
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  2. Meng Chen
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Correspondence to Meng Chen .

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Editors and Affiliations

  1. Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, CA, USA

    Meng Chen

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Fan, D., Chen, M. (2024). Dissection of Daytime and Nighttime Thermoresponsive Hypocotyl Elongation in Arabidopsis. In: Chen, M. (eds) Thermomorphogenesis. Methods in Molecular Biology, vol 2795. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3814-9_2

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  • DOI: https://doi.org/10.1007/978-1-0716-3814-9_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3813-2

  • Online ISBN: 978-1-0716-3814-9

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