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Long-Read Metagenomics and CAZyme Discovery

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Carbohydrate-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2657))

Abstract

Microorganisms play a primary role in regulating biogeochemical cycles and are a valuable source of enzymes that have biotechnological applications, such as carbohydrate-active enzymes (CAZymes). However, the inability to culture the majority of microorganisms that exist in natural ecosystems restricts access to potentially novel bacteria and beneficial CAZymes. While commonplace molecular-based culture-independent methods such as metagenomics enable researchers to study microbial communities directly from environmental samples, recent progress in long-read sequencing technologies are advancing the field. We outline key methodological stages that are required as well as describe specific protocols that are currently used for long-read metagenomic projects dedicated to CAZyme discovery.

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Authors and Affiliations

  1. Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway

    Alessandra Ferrillo, Arturo Vera-Ponce de León, Sabina Leanti La Rosa, Phillip Byron Pope & Live Heldal Hagen

  2. Faculty of Bioscience, Norwegian University of Life Sciences, Aas, Norway

    Carl Mathias Kobel, Arturo Vera-Ponce de León & Phillip Byron Pope

  3. Luxembourg Centre for Systems Biomedicine, Esch-sur-Alzette, Luxembourg

    Benoit Josef Kunath

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  1. Alessandra Ferrillo
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  2. Carl Mathias Kobel
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  3. Arturo Vera-Ponce de León
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  4. Sabina Leanti La Rosa
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  5. Benoit Josef Kunath
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  6. Phillip Byron Pope
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  7. Live Heldal Hagen
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Correspondence to Live Heldal Hagen .

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Editors and Affiliations

  1. Agriculture and Agri-Food Canada, Lethbridge Research and Development Center, Lethbridge, AB, Canada

    D. Wade Abbott

  2. Department of Biochemistry, The University of British Columbia, Kelowna, BC, Canada

    Wesley F. Zandberg

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Ferrillo, A. et al. (2023). Long-Read Metagenomics and CAZyme Discovery. In: Abbott, D.W., Zandberg, W.F. (eds) Carbohydrate-Protein Interactions. Methods in Molecular Biology, vol 2657. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3151-5_19

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  • DOI: https://doi.org/10.1007/978-1-0716-3151-5_19

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  • Publisher Name: Humana, New York, NY

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  • Online ISBN: 978-1-0716-3151-5

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