SpringerLink
Log in

Improved physicochemical pretreatment and enzymatic hydrolysis of rice straw for bioethanol production by yeast fermentation

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

Lignocellulosic biomass such as agricultural and forest residues are considered as an alternative, inexpensive, renewable, and abundant source for fuel ethanol production. In the present study, three different pretreatment methods for rice straw were carried out to investigate the maximum lignin removal for subsequent bioethanol fermentation. The chemical pretreatments of rice straw were optimized under different pretreatment severity conditions in the range of 1.79–2.26. Steam explosion of rice straw at 170 °C for 10 min, sequentially treated with 2% (w/v) KOH (SEKOH) in autoclave at 121 °C for 30 min, resulted in 85 ± 2% delignification with minimum sugar loss. Combined pretreatment of steam explosion and KOH at severity factor (SF 3.10) showed improved cellulose fraction of biomass. Furthermore, enzymatic hydrolysis at 30 FPU/g enzyme loading resulted in 664.0 ± 5.39 mg/g sugar yield with 82.60 ± 1.7% saccharification efficiency. Consequently, the hydrolysate of SEKOH with 58.70 ± 1.52 g/L sugars when fermented with Saccharomyces cerevisiae OBC14 showed 26.12 ± 1.24 g/L ethanol, 0.44 g/g ethanol yield with 87.03 ± 1.6% fermentation efficiency.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Abraham A, Mathew AK, Sindhu R, Pandey A, Binod P (2016) Potential of rice straw for bio-refining: an overview. Bioresour Technol 215:29–36

    Article  CAS  Google Scholar 

  • Agudelo RA, Garcia-Aparicio MP, Görgens JF (2016) Steam explosion pretreatment of triticale (X Triticosecale Wittmack) straw for sugar production. N Biotechnol 33:153–163

    Article  CAS  Google Scholar 

  • Ashoor S, Comitini F, Ciani Maurizio (2015) Cell-recycle batch process of Scheffersomyces stipites and Saccharomyces cerevisiae co-culture for second generation bioethanol production. Biotechnol Lett 37:2213–2218

    Article  CAS  Google Scholar 

  • Barsberg S, Selig MJ, Felby C (2013) Impact of lignins isolated from pretreated lignocelluloses on enzymatic cellulose saccharification. Biotechnol Lett 35:189–195

    Article  CAS  Google Scholar 

  • Binod P, Sindhu R, Singhania RR, Vikram S, Devi L, Nagalakshmi S, Kurien N, Sukumaran RK, Pandey A (2010) Bioethanol production from rice straw: an overview. Bioresour Technol 101:4767–4774

    Article  CAS  Google Scholar 

  • Binod P, Kuttiraja M, Archana M, Janu KU, Sindhu R, Sukumaran RK, Pandey A (2012) High temperature pretreatment and hydrolysis of cotton stalk for producing sugars for bioethanol production. Fuel 92:340–345

    Article  CAS  Google Scholar 

  • Cha YL, Yang J, Ahn JW, Moon YH, Yoon YM, Yu GD, An GH, Choi IH (2014) The optimized CO2-added ammonia explosion pretreatment for bioethanol production from rice straw. Bioprocess Biosyst Eng 37:1907–1915

    Article  CAS  Google Scholar 

  • Chandrakant P, Bisaria VS (1998) Simultaneous bioconversion of cellulose and hemicellulose to ethanol. Crit Rev Biotechnol 18:295–331

    Article  CAS  Google Scholar 

  • Chaturvedi V, Verma P (2013) An overview of key pretreatment processes employed for bioconversion of lignocellulosic biomass into biofuels and value added products. 3. Biotech 3:415–431

    Google Scholar 

  • Clairotte M, Adam TW, Zardini AA, Manfredi U, Martini G, Krasenbrink A, Vicet A, Tournie E, Astorga C (2013) Effects of low temperature on the cold start gaseous emissions from light duty vehicles fuelled by ethanol-blended gasoline. Appl Energy 102:44–54

    Article  CAS  Google Scholar 

  • El-Zawawya WK, Ibrahima MM, Abdel-Fattahb YR, Solimanb NA, Mahmoudc MM (2011) Acid and enzyme hydrolysis to convert pretreated lignocellulosic materials into glucose for ethanol production. Carbohyd Polym 84:865–871

    Article  Google Scholar 

  • Ghose TK (1987) Measurement of cellulsase activities, (recommendations of commission on biotechnology IUPAC). Pure Appl Chem 59:257–268

    CAS  Google Scholar 

  • Govumoni SP, Koti S, Kothagouni SY, Venkateshwar S, Linga VR (2013) Evaluation of pretreatment methods for enzymatic saccharification of wheat straw for bioethanol production. Carbohyd Polym 91:646–650

    Article  CAS  Google Scholar 

  • Hidalgo BQ, Monsalve-Marin F, Narvaez-Rincon PC, Pedroza-Rodriguez AM, Velasquez-Lozano ME (2013) Ethanol production by Saccharomyces cerevisiae using lignocellulosic hydrolysate from Chrysanthemum waste degradation. World J Microbiol Biotechnol 29:459–466

    Article  Google Scholar 

  • Ibrahima MM, E-Zawawya WK, Abdel-Fattahb YR, Solimanb NA, Agblevor FA (2011) Comparison of alkaline pul** with steam explosion for glucose production from rice straw. Carbohyd Polym 83:720–726

    Article  Google Scholar 

  • Jiang Z, Fei B, Li Z (2016) Pretreatment of bamboo by ultra-high pressure explosion with a high pressure homogenizer for enzymatic hydrolysis and ethanol fermentation. Bioresour Technol 214:876–880

    Article  CAS  Google Scholar 

  • ** SY, Chen HZ (2006) Superfine grinding of steam-exploded rice straw and its enzymatic hydrolysis. Biochem Eng J 30:225–230

    Article  CAS  Google Scholar 

  • Kafle K, Lee CM, Shin H, Zoppe J, Johnson DK, Kim SH, Park S (2015) Effects of delignification on crystalline cellulose in lignocellulose biomass characterized by vibrational sum frequency generation spectroscopy and X-ray diffraction. Bioenerg Res 8:1750–1758

    Article  CAS  Google Scholar 

  • Karuna N, Zhang L, Walton JH, Couturier M, Oztop MH, Master ER, McCarthy MJ, Jeoh T (2014) The impact of alkali pretreatment and post-pretreatment conditioning on the surface properties of rice straw affecting cellulose accessibility to cellulases. Bioresour Technol 167:232–240

    Article  CAS  Google Scholar 

  • Keshav PK, Naseeruddin S, Rao LV (2016a) Improved enzymatic saccharification of steam exploded cotton stalk using alkaline extraction and fermentation of cellulosic sugars into ethanol. Bioresour Technol 214:363–370

    Article  CAS  Google Scholar 

  • Keshav PK, Shaik N, Koti S, Linga VR (2016b) Bioconversion of alkali delignified cotton stalk using two-stage dilute acid hydrolysis and fermentation of detoxified hydrolysate into ethanol. Indus Crops Prod 91:323–331

    Article  CAS  Google Scholar 

  • Kim Y, Kreke T, Mosier NS, Ladisch MR (2014) Severity factor coefficients for subcritical liquid hot water pretreatment of hardwood chips. Biotechnol Bioeng 111:254–263

    Article  Google Scholar 

  • Kuhad RC, Gupta R, Khasa YP, Singh A (2010) Bioethanol production from Lantana camara (red sage): pretreatment, saccharification and fermentation. Bioresour Technol 101:8348–8354

    Article  CAS  Google Scholar 

  • Liu X, Ma Y, Zhang M (2015) Research advances in expansins and expansion-like proteins involved in lignocellulose degradation. Biotechnol Lett 37:1541–1551

    Article  CAS  Google Scholar 

  • Martinez A, Rodriguez ME, York SW, Preston JF, Ingram LO (2000) Effect of Ca(OH)2 treatments (“Over liming”) on the composition and toxicity of bagasses hemicellulose hydrolysate. Biotechnol Bioeng 69:526–536

    Article  CAS  Google Scholar 

  • Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  • Montane D, Farriol X, Salvado J, Jollez P, Chornet E (1998) Fractionation of wheat straw by steam-explosion pretreatment and alkali delignification. Cellulose pulp and byproducts from hemicellulose and lignin. J Wood Chem Technol 18:171–191

    Article  CAS  Google Scholar 

  • Nakamura Y, Sawada T, Inoue E (2001) Enhanced ethanol production from enzymatically treated steam-exploded rice straw using extractive fermentation. J Chem Technol Biotechnol 76:879–884

    Article  CAS  Google Scholar 

  • Naseeruddin S, Yadav KS, Sateesh L, Manikyam A, Desai S, Rao LV (2013) Selection of the best chemical pretreatment for lignocellulosic substrate Prosopis juliflora. Bioresour Technol 136:542–549

    Article  CAS  Google Scholar 

  • Petrik S, Kadar Z, Marova I (2013) Utilization of hydrothermally pretreated wheat straw for production of bioethanol and carotene-enriched biomass. Bioresour Technol 133:370–377

    Article  CAS  Google Scholar 

  • Silverstein RA, Chen Y, Sharma-Shivappa RR, Boyette MD, Osborne J (2007) A comparison of chemical pretreatment methods for improving saccharification of cotton stalks. Bioresourc Technol 98:3000–3011

    Article  CAS  Google Scholar 

  • Sindhu R, Kuttiraja M, Prabisha TP, Binod P, Sukumaran RK, Pandey A (2016) Development of a combined pretreatment and hydrolysis strategy of rice straw for the production of bioethanol and biopolymer. Bioresour Technol 215:110–116

    Article  CAS  Google Scholar 

  • Singleton VL, Orthofer R, Lauelaravetos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299:152–178

    Article  CAS  Google Scholar 

  • Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2008) Determination of structural carbohydrates and lignin in biomass. Lab Anal Proced 1617:1–16

    Google Scholar 

  • Sun SL, Wen JL, Ma MG, Sun RC (2014) Enhanced enzymatic digestibility of bamboo by a combined system of multiple steam explosion and alkaline treatments. Appl Energy 136:519–526

    Article  CAS  Google Scholar 

  • Thakur S, Shrivastava B, Ingale S, Kuhad RC, Gupte A (2013) A degradation and selective lignin lysis of wheat straw and banana stem for an efficient bioethanol production using fungal and chemical pretreatment. Biotech 3:365–372

    Google Scholar 

  • Tiwari R, Nain L, Labrou NE, Shukla P (2017) Bioprospecting of functional cellulases from metagenome for second generation biofuel production: a review. Crit Rev Microbiol 13:1–14

    Article  Google Scholar 

  • Wi SG, Choi IS, Kim KH, Kim HM, Bae HJ (2013) Bioethanol production from rice straw by pop** pretreatment. Biotechnol Biofuels 6:166

    Article  Google Scholar 

  • Wiater MK, Targonski Z (2002) Ethanol fermentation on glucose/xylose mixture by co-cultivation of restricted glucose catabolite repressed mutants of Pichia stipitis with respiratory deficient mutants of Saccharomyces cerevisiae. Acta Microbiol Pol 51:345–352

    Google Scholar 

  • Yu G, Yano S, Inoue H, Inoue S, Endo T, Sawayama S (2010) Pretreatment of rice straw by a hot-compressed water process for enzymatic hydrolysis. Appl Biochem Biotechnol 160:539–551

    Article  CAS  Google Scholar 

  • Zhang M, Shukla P, Ayyachamy M, Permaul K, Singh S (2010) Improved bioethanol production through simultaneous saccharification and fermentation of lignocellulosic agricultural wastes by Kluyveromyces marxianus 6556. World J Microbiol Biotechnol 26:1041–1046

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Authors are grateful to the University Grants Commission (UGC), New Delhi, Govt. of India for financial support under UPE program and for providing research fellowship under basic science research and research fellowship for science meritorious students (BSR-RFSMS) program.

Author information

Authors and Affiliations

  1. Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana, 500007, India

    Chandrasekhar Banoth, Bindu Sunkar, Pruthvi Raj Tondamanati & Bhima Bhukya

Authors
  1. Chandrasekhar Banoth
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bindu Sunkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pruthvi Raj Tondamanati
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bhima Bhukya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bhima Bhukya.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Consent for publication

This article does not contain any individual person’s data.

Declaration

We declare that this manuscript has not been published elsewhere and is not under consideration by another journal. All authors have approved the final manuscript and agreed with the submission to “3 Biotech”.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Banoth, C., Sunkar, B., Tondamanati, P.R. et al. Improved physicochemical pretreatment and enzymatic hydrolysis of rice straw for bioethanol production by yeast fermentation. 3 Biotech 7, 334 (2017). https://doi.org/10.1007/s13205-017-0980-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-017-0980-6

Keywords

Search

Navigation