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Anaerotignum faecicola sp. nov., isolated from human faeces

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

A strictly anaerobic bacterium, designated as strain KGMB-03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status. Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods. The isolate grew at 10–45°C (optimum temperature of 40°C) and a pH range of 5.1–10.5 (optimum pH of 6.8). Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%). The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3–71.0%, which are below the ANI criterion for interspecies identity. The DNA G + C content based on the whole-genome sequence is 47.3 mol%. The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0. Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan. Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp. nov. is proposed. The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).

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References

  • Browne, H.P., Forster, S.C., Anonye, B.O., Kumar, N., Neville, B.A., Stares, M.D., Goulding, D., and Lawley, T.D. 2016. Culturing of ‘unculturable’ human microbiota reveals novel taxa and extensive sporulation. Nature533, 543–546.

    Article  CAS  Google Scholar 

  • Chun, J. and Goodfellow, M. 1995. A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int. J. Syst. Bacteriol.45, 240–245.

    Article  CAS  Google Scholar 

  • Euzeby, J.P. 1997. List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol.47, 590–592.

    Article  CAS  Google Scholar 

  • Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol.17, 368–376.

    Article  CAS  Google Scholar 

  • Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Biol.20, 406–416.

    Article  Google Scholar 

  • Guinane, C.M. and Cotter, P.D. 2013. Role of the gut microbiota in health and chronic gastrointestinal disease: understanding a hidden metabolic organ. Therap. Adv. Gastroenterol.6, 295–308.

    Article  Google Scholar 

  • Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol.16, 111–120.

    Article  CAS  Google Scholar 

  • Kumar, S., Stecher, G., and Tamura, K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol. Biol. Evol.33, 1870–1874.

    Article  CAS  Google Scholar 

  • Lee, I., Kim, Y.O., Park, S.C., and Chun, J. 2016. OrthoANI: An improved algorithm and software for calculating average nucleotide identity. Int. J. Syst. Evol. Microbiol.66, 1100–1103.

    Article  CAS  Google Scholar 

  • Lozupone, C.A., Stombaugh, J.I., Gordon, J.I., Jansson, J.K., and Knight, R. 2012. Diversity, stability and resilience of the human gut microbiota. Nature489, 220–230.

    Article  CAS  Google Scholar 

  • McFall-Ngai, M., Hadfield, M.G., Bosch, T.C., Carey, H.V., Domazet-Loso, T., Douglas, A.E., Dubilier, N., Eberl, G., Fukami, T., Gilbert, S.F., et al. 2013. Animals in a bacterial world, a new imperative for the life sciences. Proc. Natl. Acad. Sci. USA110, 3229–3236.

    Article  CAS  Google Scholar 

  • Meehan, C.J. and Beiko, R.G. 2014. A phylogenomic view of ecological specialization in the Lachnospiraceae, a family of digestive tract-associated bacteria. Genome Biol. Evol.6, 703–713.

    Article  CAS  Google Scholar 

  • Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol.4, 406–425.

    CAS  PubMed  Google Scholar 

  • Schleifer, K.H. and Kandler, O. 1972. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev.36, 407–477.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Stackebrandt, E. 2014. The Family Lachnospiraceae. In Rosenberg, E., DeLong, E.F., Lory, S., Stackebrandt, E., and Thompson, F. (eds.), The Prokaryotes: Firmicutes and Tenericutes, pp. 197–201. Springer Berlin Heidelberg, Berlin, Heidelberg, Germany.

    Google Scholar 

  • Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res.25, 4876–4882.

    Article  CAS  Google Scholar 

  • Tittsler, R.P. and Sandholzer, L.A. 1936. The use of semi-solid agar for the detection of bacterial motility. J. Bacteriol.31, 575–580.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ueki, A., Goto, K., Ohtaki, Y., Kaku, N., and Ueki, K. 2017. Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum. Int. J. Syst. Evol. Microbiol.67, 4146–4153.

    Article  CAS  Google Scholar 

  • Van der Wielen, P.W., Rovers, G.M., Scheepens, J.M., and Biesterveld, S. 2002. Clostridium lactatifermen tans sp. nov., a lactateermenting anaerobe isolated from the caeca of a chicken. Int. J. Syst. Evol. Microbiol.52, 921–925.

    PubMed  Google Scholar 

  • Yoon, S.H., Ha, S.M., Kwon, S., Lim, J., Kim, Y., Seo, H., and Chun, J. 2017. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int. J. Syst. Evol. Microbiol.67, 1613–1617.

    Article  CAS  Google Scholar 

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Acknowledgements

We thank Professor Bernhard Schink for his advice on naming the novel strain. This research was supported by the Bio & Medical Technology Development program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MIST) of the Republic of Korea and a grant from the Korea Research Institute of Bioscience & Biotechnology (KRIBB) Research Initiative Program. J.-S. L. was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2016M3A9F3946674). S.-H.P. was supported by the Basic Science Research Program through the NRF funded by the Ministry of Education (NRF-2014R1A6A3A-04057492). This study was conducted in accordance with the IRB regulation at KCTC (P01-201702-31-007).

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  1. These authors contributed equally to this study

Authors and Affiliations

  1. Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 56212, Republic of Korea

    Seung-Hyeon Choi, Ji-Sun Kim, Jam-Eon Park, Keun Chul Lee, Mi Kyung Eom, Byeong Seob Oh, Seung Yeob Yu, Se Won Kang, Kook-Il Han, Min Kuk Suh, Ju Huck Lee, Jung-Sook Lee & Seung-Hwan Park

  2. Seoul National University Bundang Hospital, Seongnam, 13620, Republic of Korea

    Dong Ho Lee & Hyuk Yoon

  3. ChunLab, Inc., Seoul, 06725, Republic of Korea

    Byung-Yong Kim & Je Hee Lee

  4. University of Science and Technology (UST), Daejeon, 34113, Republic of Korea

    Jung-Sook Lee

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  1. Seung-Hyeon Choi
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Corresponding authors

Correspondence to Jung-Sook Lee or Seung-Hwan Park.

Additional information

Supplemental material for this article may be found at http://www.springerlink.com/content/120956.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KGMB03357T is MK439508.

The GenBank/EMBL/DDBJ accession number for the whole-genome sequence of strain KGMB03357T is BHVZ00000000.

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Choi, SH., Kim, JS., Park, JE. et al. Anaerotignum faecicola sp. nov., isolated from human faeces. J Microbiol. 57, 1073–1078 (2019). https://doi.org/10.1007/s12275-019-9268-3

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  • DOI: https://doi.org/10.1007/s12275-019-9268-3

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